中华皮肤科杂志 ›› 2004, Vol. 37 ›› Issue (1): 25-26.

• 论著 • 上一篇    下一篇

系统性红斑狼疮患者红细胞Ⅰ型补体受体表达及基因型的研究

彭学标1, 汪一琴2   

  1. 1. 第一军医大学南方医院皮肤科, 广州510515;
    2. 广州市钟落潭医院
  • 收稿日期:2003-02-10 出版日期:2004-01-15 发布日期:2004-01-15
  • 基金资助:
    广东省自然科学基金资助项目(001082)

Genotype and Quantitative Expression of Type ⅠComplement Receptor on Erythrocytes from the Patients with Systemic Lupus Erythematosus

PENG Xue-biao1, WANG Yi-qin2   

  1. Department of Dematology, Nan Fang Hospital, First Millitary Medical University, Guangzhou 510515, China
  • Received:2003-02-10 Online:2004-01-15 Published:2004-01-15

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摘要： 目的 研究系统性红斑狼疮(SLE)患者红细胞Ⅰ型补体受体(CR1)基因型及数量表达,分析CR1与病情发展的关系。方法 采用PCR和HindⅢ酶切技术作CR1基因型分析,采用流式细胞仪测定CR1的数量表达。结果 与正常人对照组相比,SLE患者红细胞CR1分子高表达基因型明显降低,而低表达基因型明显升高;SLE患者红细胞CR1数量表达明显降低,与SLEDAI呈负相关,且活动期低于非活动期。结论 红细胞CR1分子的表达与SLE病情活动密切相关,对SLE患者进行红细胞CR1分子的定量测定,对病情判断有参考价值。

关键词: 红斑狼疮,系统性, 受体,补体, 红细胞

Abstract: Objective Through studing the genotype and quantitatively analysing expression of type Ⅰ complement receptor(CR1) on erythrocytes from the patients with systemic lupus erythematosus(SLE). To investigate the relationship between CR1 and SLE. Methods Polymerase chain reaction(PCR) and Hind Ⅲ restriction enzyme digestion were used to analyse the genotype of CR1, and the quantitative expression of CR1 was demonstrated by FACscan flow cytometry. Results Compared with normal controls, high expression genotype of CR1 was significantly decreased, but low expression genotype was significantly increased in SLE patients. The number of CR1 on erythrocytes in SLE patients was significantly lower than that in normal controls. There was a negative relationship between the expression of CR1 and disease activity index of SLE (SLEDAI). Conclusion The expression of CR1 on erythrocytes might be related to the development of SLE, and it is helpful to detect the CR1 on erythrocytes for the determination of SLE patient's condition.

Key words: Lupus erythematosus, systemic, Receptors, complement, Erythrocytes