中华皮肤科杂志 ›› 2003, Vol. 36 ›› Issue (9): 496-498.

• 论著 • 上一篇    下一篇

博来霉素诱导硬皮病小鼠模型及其抗体测定的初步研究

朱鹭冰, 李明   

  1. 复旦大学附属中山医院皮肤科, 上海200032
  • 收稿日期:2002-09-20 出版日期:2003-09-15 发布日期:2003-09-15

Establishment and Antibody Verification of the Animal Model of Sclerotic Skin Induced by Bleomycin

ZHU Lu-bing, LI Ming   

  1. Zhong Shan Hospital Affiliated to Fu Dan University, Shanghai 200032, China
  • Received:2002-09-20 Online:2003-09-15 Published:2003-09-15

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摘要： 目的 以博来霉素(Blm)诱导建立硬皮病小鼠模型并加以验证。方法 将200μg/mLBlm100μL注射在C3H/He小鼠(C3H小鼠)背部皮下,每日1次,连续3周,建立硬皮病小鼠模型,并经皮肤、肺组织病理学及血清自身抗体检测加以验证。结果 注射200μg/mLBlm 3周的C3H小鼠,与对照组相比,注射区皮肤厚度明显增加(P<0.001),胶原纤维组织化学指数显著升高(P<0.0001),真皮出现均匀硬化表现;该小鼠肺泡间隔增厚,伴单一核细胞浸润,并可见肺组织小血管壁增厚;血清ANA阳性,间接免疫荧光法检测见细胞核呈粗斑点或细斑点状黄绿色荧光,有的斑点相互聚集成若干大斑点位于核中央,结合抗ENA抗体测定提示存在抗Scl-70抗体、抗着丝点抗体和抗U1-RNP抗体等。结论 对C3H小鼠背部每日皮下注射200μg/mLBlm共3周,成功建立了硬皮病小鼠模型。

关键词: 硬皮病,系统性, 模型,动物, 博来霉素, 抗体,抗核

Abstract: Objective To establish and verify the animal model of sclerotic skin induced by bleomycin(Blm).Methods To establish a mouse model for scleroderma in C3H/He mice by repeated local injection of 100μL of Blm(200μg/mL)everyday for 3 weeks.Then,the specimens of skin,lung and serum were examined.Results After 3 week local Blm injections,an intense dermal sclerosis was shown in C3H/He mice.Compared with the control skin,increased dermal thickness and increased collagen histo-chemical index were found(P<0.001,P<0.0001).The examination of the lung of mice revealed thick-ened alveolar septa with infiltration of mononuclear cells and thickening of vascular walls.The speckled and nucleolar ANA patterns were detected.Anti-U1RNP,anti-Scl-70 and anticentromere antibodies were identi-fied together with the results of immunoblot.Conclusion Local injections of Blm(200μg/mL)for 3 weeks successfully induced sclerotic skin mimicking scleroderma in C3H mice.

Key words: Scleroderma,systemic, Models,animal, Bleomycin, Antibodies,antinuclear