中华皮肤科杂志 ›› 2003, Vol. 36 ›› Issue (8): 446-448.

• 论著 • 上一篇    下一篇

念珠菌性外阴阴道炎患者身体不同部位白念珠菌基因型研究

朱晓芳1, 汪清2, 章强强1, 任大明3, 王家俊1   

  1. 1. 复旦大学附属华山医院皮肤科 上海 200040;
    2. 复旦大学附属妇产科医院;
    3. 复旦大学遗传所
  • 收稿日期:2002-08-01 出版日期:2003-08-15 发布日期:2003-08-15

Genotyping of Candida albicans Isolated from Different Body Sites of Women Suffering from Candidal Vulvovaginitis

ZHU Xiao-fang1, WANG Qing2, ZHANG Qiang-qiang1, REN Da-ming3, WANG Jia-jun1   

  1. Huashan Hospital, Medical College of Fudan University, Shanghai 200040, China
  • Received:2002-08-01 Online:2003-08-15 Published:2003-08-15

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摘要： 目的 研究念珠菌性外阴阴道炎患者身体不同部位的白念珠菌基因型分布情况。方法 采用PCR方法扩增白念珠菌包含Ⅰ类内含子的编码rRNA的25SrDNA区,根据扩增条带大小进行基因分型:A型(~450bp),B型(~840bp),C型(~450bp和~840bp)。结果 60例念珠菌性外阴阴道炎患者中,54例由白念珠菌感染所致,其中表现为非复发性感染者39例,复发性感染者15例;同时从2个或2个以上部位分离到白念珠菌的病例共32例(32/54),其中非复发性念珠菌性外阴阴道炎19例(19/39),复发性念珠菌性外阴阴道炎13例(13/15)。从54例白念珠菌性外阴阴道炎患者的阴道、肛门、口腔部位共分离到92株白念珠菌,其中A型80株,B型8株,C型4株。同一患者不同部位基因型相同者24例(24/32),不同者8例(8/32)。结论 在白念珠菌性外阴阴道炎中,主要以A型白念珠菌为主,B型、C型所占比例较少,且多分离自非阴道部位。在复发性念珠菌性外阴阴道炎患者中,身体其他部位念珠菌的定植率高于非复发性念珠菌性外阴阴道炎,肠道菌库理论在念珠菌性外阴阴道炎的反复发作中,具有一定的作用。

关键词: 念珠菌病,外阴阴道, 念珠菌,白色, 基因型

Abstract: Objective To study the distribution of genotypes of Candida albicans isolated from different body sites of patients with candidal vulvovaginitis(CVV).Methods PCR was designed to amplify group I intron-containing region in25S rDNA of Candida albicans.The strains of Candida albicans could be classified into three genotypes:genotype A(~450bp),B(~840bp)and C(~450bp and ~840bp),on the basis of different ranges of bands of amplicons.Results Sixty women with CVV were recruited,of whom54were caused by Candida albicans.Among the54patients 39 had non-recurrent CVV and 15 had recurrent CVV(RCVV).Candida albicans could be isolated simultaneously from different body sites in32of54patients,including 19(19/39) with non-RCVV and 13(13/15) with RCVV.A total of 92 strains of Candida albicans were isolated from vagina,tongue and anus in54patients with CVV.Eighty strains of genotype A,8of genotype B and4of genotype C were found.The same genotypes of Candida albicans in different body sites were identified in24patients,and the different genotypes were identified in 8 patients.Conclusion Genotype A is predominant in CVV.The other two genotypes(B and C)are not commonly seen,and mainly isolated from non-vaginal sites.The colonization of Candida albicans in the non-vagina sites is more frequent in RCVV than that in CVV,and the intestinal reservoir theory may play a role in the relapse of RCVV.

Key words: Candidiasis,vulvovaginal, Candida albicans, Genotype