从花斑癣患者的皮损区及非皮损区分离和鉴定马拉色菌

中华皮肤科杂志 ›› 2003, Vol. 36 ›› Issue (8): 430-432.

从花斑癣患者的皮损区及非皮损区分离和鉴定马拉色菌

李志瑜, 冉玉平, 熊琳, 代亚林, 郭晓莉

四川大学华西医院皮肤性病科

收稿日期:2002-08-01 出版日期:2003-08-15 发布日期:2003-08-15
通讯作者: 冉玉平,四川大学华西医院皮肤性病科,成都,610041
基金资助:
四川省学术和技术带头人培养基金(4989904)

Isolation and Identification of Malassezia Species from Lesional and Non-lesional Sites of Patients with Pityriasis Versicolor

LI Zhi-yu, RAN Yu-ping, XIONG Lin, DAI Ya-lin, GUO Xiao-li

Department of Dermatovenereology, West China Hospital, Sichuan University, Chengdu 610041, China

Received:2002-08-01 Online:2003-08-15 Published:2003-08-15

摘要/Abstract

摘要： 目的研究花斑癣患者皮损区及非皮损区马拉色菌菌种构成;不同解剖部位、皮损颜色及各菌种的分布情况;患者病情和年龄与菌种构成的关系。方法用无菌胶带粘取113例花斑癣患者皮损区及非皮损区共629个部位的皮屑,分别接种于含菜子油培养基中分离马拉色菌,用生理生化及形态学方法鉴定菌种。结果皮损区与相对应的非皮损区马拉色菌分离阳性率无差别,非皮损区额部和胸背部分离阳性率高于上、下肢。共分离到565株马拉色菌,鉴定出合轴马拉色菌(44.78%)、糠秕马拉色菌(32.94%)、球形马拉色菌(11.68%)、钝形马拉色菌(5.84%)及限制马拉色菌(4.76%)共5个种,有27处(5.01%)同时分离到两种菌。皮损区与非皮损区菌种构成无明显差别,限制马拉色菌主要从额部分离出。菌种构成与皮损面积无关,但与皮损颜色和患者年龄有关。皮损颜色与病程无关。结论花斑癣患者皮损区与非皮损区马拉色菌分离阳性率和菌种构成基本一致,与病情无关,而不同解剖部位、皮损类型及年龄患者的菌种构成有一定差异。

关键词: 花斑癣, 马拉色霉菌属

Abstract: Objective To investigate the distribution of Malassezia species in lesional and non-lesional sites of patients with pityriasis versicolor(PV),species-variation in different anatomic sites and in lesions with different pigmentation,and the relationship between various Malassezia species and severity and age of PV patients.Methods A total of629skin specimens taken by sterile adhesive tape from the lesions and non-lesional skin were inoculated on media containing rapeseed oil in113patients with PV.Isolated colonies were identified to species based on physiological and morphological characteristics.Results The isolation rates of Malassezia spp.were not significantly different from both lesions and corresponding non-lesional skin.Among non-lesional sites,the isolation rate was significantly higher in forehead and trunk than that in upper and lower extremities.Five species were identified out of565strains obtained from the patients,including M.sympodialis(44.78%),M.furfur(32.94%),M.globosa(11.68%),M.obtusa(5.84%)and M.restricta(4.76%).Two different species were isolated simultaneously from27sites.There was no obvious difference in species distribution patterns between lesions and non-lesional sites.M.restricta was isolated from forehead exclusively.Species-variation was closely linked to lesions with different pigmentation and the age of patients,not to the severity of disease.Conclusion There is neither statistical difference of Malassezia isolation rate and species distribution between lesions and non-lesional skin,nor correlation between disease severity and species-variation.The anatomic sites,the diversity of pigmentation pattern and the age of patients seem to be associated with different Malassezia species.

Key words: Tinea versicolor, Malassezia

李志瑜, 冉玉平, 熊琳, 代亚林, 郭晓莉. 从花斑癣患者的皮损区及非皮损区分离和鉴定马拉色菌[J]. 中华皮肤科杂志, 2003, 36(8): 430-432.

LI Zhi-yu, RAN Yu-ping, XIONG Lin, DAI Ya-lin, GUO Xiao-li. Isolation and Identification of Malassezia Species from Lesional and Non-lesional Sites of Patients with Pityriasis Versicolor[J]. Chinese Journal of Dermatology, 2003, 36(8): 430-432.

参考文献

[1] Galadari I, el Komy M, Mousa A, et al. Tinea versicolor:histologic and ultrastructural investigation of pigmentary changes. Int J Dermatol, 1992, 31:253-256.
[2] Gueho E, Midgley G, Guillot J. The genus Malassezia with description of four new species. Antonie Van Leeuwenhoek, 1996, 69:337-355.
[3] Crespo Erchiga V, Ojeda Martos A, Vera Casano A, et al. Malassezia globosa as the causative agent of pityriasis versicolor. Br J Dermatol, 2000, 143:799-803.
[4] 冉玉平,罗汉超,李志玉.含菜子油培养基对花斑癣致病真菌的培养研究.中华皮肤科杂志,1987,20:4-7.
[5] 熊琳,冉玉平,周光平,等.马拉色菌属的分类及其在几种皮肤病的分布情况.中华皮肤科杂志,2000,33:318-320.
[6] 冉玉平.检测糠秕马拉色菌的几种取材染色和培养方法介绍.中国皮肤性病学杂志,1999,13:181-182.
[7] Guillot G, Gueho E, Lesourd M, et al. Identification of Malassezia species:a practical approach. J Mycol Med, 1996, 6:103-110.
[8] Mayser P, Haze P, Papavassilis C, et al. Differentiation of Malassezia species:selectivity of cremophor EL, castor oil and ricinoleic acid for M. furfur. Br J Dermatol, 1997, 137:208-213.
[9] 路永红,冉玉平,熊琳,等.从夫妻双方的头皮屑中分离和鉴定马拉色菌.中华皮肤科杂志,2002,35:369-371.
[10] Gupta AK, Kohli Y, Summ erbell RC, et al. Quantitative culture of Malassezia species from different body sites of individuals with or without dermatoses. Med Mycol, 2001, 39:243-251.
[11] Nakabayashi A, Sei Y, Guillot J. Identification of Malassezia species isolated from patients with seborrhoeic dermatitis, atopic dermatitis, pityriasis versicolor and normal subjects. Med Mycol,2000, 38:337-341.
[12] Aspiroz C, Moreno LA, Rezusta A, et al. Differentiation of three biotypes of Malassezia species on human normal skin. correspondence with M. globosa, M. sympodialis and M. restricta. Mycopathologia, 1999, 145:69-74.