中华皮肤科杂志 ›› 2003, Vol. 36 ›› Issue (6): 316-317.

• 论著 • 上一篇    下一篇

角质形成细胞对MHC-Ⅰ限制的自身抗原呈递作用的体外研究

高兴华1, 小阪博2, 徐海燕1, 赵玉铭1, 陈洪铎1, 吉川邦彦2   

  1. 1. 中国医科大学第一临床学院皮肤科 沈阳 110001;
    2. 日本大阪大学医学部皮肤科
  • 收稿日期:2002-04-05 出版日期:2003-06-15 发布日期:2003-06-15
  • 通讯作者: 陈洪铎,110001 沈阳,中国医科大学附属第一医院皮肤科,Email:hongduochen@hotmail.com E-mail:hongduochen@hotmail.com
  • 基金资助:
    国家自然科学基金资助课题(39970682)

Presentation of an Endogenous MHC ClassⅠRestricted Autoantigen by Keratinocytes in Vitro

GAO Xing-hua1, Hiroshi Kosaka2, XU Hai-yan1, ZHAO Yu-ming1, CHEN Hong-duo1, Kunihiku Yoshikawa2   

  1. Department of Dermatology, First Affiliated Hospital of China Medical University, Shenyang 110001, China
  • Received:2002-04-05 Online:2003-06-15 Published:2003-06-15

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摘要： 目的 探讨角质形成细胞对抗原受体特异的CD8阳性T细胞的抗原呈递作用。方法 利用已建立的转染细胞系PAM-OK(可表达H-2Kb/卵白蛋白多肽复合体,氨基酸257-264)及对照细胞系,刺激幼稚或已被预激活的卵白蛋白特异的CD8阳性T细胞(H-2Kb)作为反应细胞,以3H-胸腺嘧啶掺入法检测T细胞的增殖反应。结果 经γ干扰素刺激表达足够的抗原后,PAM-OK可刺激预激活的特异T细胞发生增殖反应,而不能刺激幼稚的T细胞发生反应。结论 角质形成细胞有使预激活的抗原特异的T细胞发生增殖的潜能,该结果有助于解释某些皮肤病局部免疫反应持续存在的现象。

关键词: 角蛋白细胞, 抗原提呈, CD8阳性T淋巴细胞

Abstract: Objective To study the antigen presenting function of keratinocytes to CD8+ T cells with specific TCR.Methods PAM212 transfectants,which express H-2Kb/ovalbumin complex,aa 257-264,were used as stimulating cells,and naive or primed CD8+ T cells with ovalbumin specific TCR were used as respon-ders.3H-thymidin uptake values were used as indicators of T cell proliferation.Results With prestimulation of IFN-γ,the proliferation of primed T cells but not naive T cells could be stimulated by PAM-OK cells.Conclusion Keratinocytes have the potential to stimulate the proliferation of TCR specific CD8+ T cells,which might explain the persistence of local immune reaction in some involved skin.

Key words: Keratinocytes, Antigen presentation, CD8-positive T-lymphocytes