中华皮肤科杂志 ›› 2003, Vol. 36 ›› Issue (5): 253-255.

• 论著 • 上一篇    下一篇

不同病程尖锐湿疣患者外周血Th细胞亚群的研究

李振1, 曾耀英2, 蔡小嫦2, 何贤辉2, 徐丽慧2, 曾山2, 狄静芳2   

  1. 1. 深圳市龙岗中心医院皮肤科, 518116;
    2. 暨南大学组织移植与免疫中心
  • 收稿日期:2002-07-24 出版日期:2003-05-15 发布日期:2003-05-15
  • 基金资助:
    国家重点基础研究发展规划项目“973”基金资助(G999054303)

Studies on the Th-cell Subsets From Peripheral Blood of the Patients with Different Courses of Condyloma Acuminatum

LI Zhen1, ZENG Yao-ying2, CAI Xiao-chang2, HE Xian-hui2, XU Li-hui2, ZENG Shan2, DI Jing-fang2   

  1. Department of Dermatology, Longgang Central Hospital, Shenzhen 518116, China
  • Received:2002-07-24 Online:2003-05-15 Published:2003-05-15

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摘要： 目的 从辅助性T细胞(Th)极化情况,探讨不同病程尖锐湿疣患者的细胞免疫状态。方法 取肝素抗凝血,用密度梯度离心法分离出血浆和外周血单一核细胞(PBMC)进行细胞培养和免疫荧光染色:PHA和自身血浆刺激PBMC诱导T细胞极化,72h后收获细胞,分别标记抗CD4-PE与抗CCR5-FITC和抗CD4-FITC与抗CCR3-PE,最后利用流式细胞仪对标记细胞进行分析。结果 尖锐湿疣患者中,短病程组与长病程组Th1/Th2极化细胞百分率分别为(25.82±2.22)%/(14.80±1.14)%与(12.20±1.37)%/(13.74±0.99)%,两组极化细胞百分率分别与正常对照组比较差异均有显著性(P<0.05),短病程组Th1/Th2的比值为1.75±0.17,而长病程组为0.89±0.11。结论 短病程尖锐湿疣患者机体呈现Th1型反应,而长病程者则以Th2型反应为主,存在Th1型反应向Th2型反应切换的现象。

关键词: 尖锐湿疣, CD4阳性T淋巴细胞

Abstract: Objective To investigate the status of cellular immunity from Th cell polarization in patients with different courses of condyloma acuminatum(CA).Methods The isolated PBMC were polarized by PHA and self-plasma for 72 hours,then followed by two-color immunofluorescent staining with anti-CD4-PE and anti-CCR5-FITC,or with anti-CD4-FITC and anti-CCR3-PE.Finally the stained cells were analyzed by flow-cytometry.Results The percentages of Th1/Th2 cells of the short-course group and long-course group were(25.82±2.22)%/(14.80±1.14)% and(12.20±1.37)%/(13.74±0.99)%,respectively;the differences between normal control and two CA groups were significant(P<0.05).The Th1/Th2 cells ratios of short-course and long-course group were1.75±0.17 or 0.89±0.11,respectively.Conclusions It seems that there was Th1 predominant response in CA patients with short-course and Th2 predominant response in long-course group.These data indicate that there may be an possible switch from Th1to Th2 response in CA patient with long-course.

Key words: Condylomata acuminata, CD4-positive T-lymphocytes