中华皮肤科杂志 ›› 2003, Vol. 36 ›› Issue (5): 248-249.

• 论著 • 上一篇    下一篇

端粒酶活性与细胞凋亡在尖锐湿疣患者组织中的表达

谢红付, 冯浩, 范学工, 施为, 陈明亮, 杜乾君, 陈翔   

  1. 中南大学湘雅医院皮肤科, 长沙410008
  • 收稿日期:2002-07-11 出版日期:2003-05-15 发布日期:2003-05-15
  • 通讯作者: 范学工,中南大学湘雅医院皮肤科, 长沙410008(E-mail:xgfan@hotmail.com) E-mail:xgfan@hotmail.com
  • 基金资助:
    湖南省科技厅资助课题(01ssy2008—14)

Telomerase Activity in Condyloma Acuminatum:Correlation with Decreased Apoptosis

XIE Hong-fu, FENG Hao, FAN Xue-gong, SHI Wei, CHEN Ming-liang, DU Qian-jun, CHEN Xiang   

  1. Xiangya Hospital Affiliated to Central South University, Changsha 410008, China
  • Received:2002-07-11 Online:2003-05-15 Published:2003-05-15

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摘要： 目的 探讨尖锐湿疣(CA)皮损中端粒酶活性与细胞凋亡表达情况及其两者之间的相关性。方法 采用端粒重复序列扩增文件-酶标法(TRAP-ELISA)检测了30例尖锐湿疣患者皮损端粒酶活性,并与正常组织和肿瘤细胞株作对照;原位末端标记法(TUNEL)检测皮损中细胞凋亡。结果 27例(90%)尖锐湿疣皮损中端粒酶活性A450值为0.50~2.76,平均1.302,明显高于正常皮肤组织,差异有显著性(t=6.12,P<0.01);24例皮损中有凋亡细胞,占80%,且端粒酶活性与凋亡指数呈显著负相关,r=-0.52,P=0.004。结论 端粒酶可以在一些非恶性皮肤病中表达,且影响细胞凋亡,可能参与CA发病。

关键词: 尖锐湿疣, 端粒,末端转移酶, 脱噬作用

Abstract: Objective To study the telomerase activity,the keratinocyte apoptosis in condyloma accuminatum(CA)and the correlation between them.Methods CA specimens from 30 patients were studied,and compared with normal tissue and tumor cell lines.Telomerase activity was detected with telomeric repeat amplification polymerase chain reaction(TRAP)-ELISA.Terminal deoxynucleotidyl transferase(TdT)-mediated biotin dUTP nick end-labeling(TUNEL)was used to evaluate apoptotic cells.Results Increased telomerase activity was detected in 27(90%)patients with CA,with A450 ranging between 0.50 and 2.76(mean 1.3022).Apoptotic keratinocytes were found in 24 out of 30 CA cases(80%).A statistically significant inverse correlation was found between telomerase activity and apoptotic index(r=-0.52,P=0.004).Conclusion Keratinocyte telomerase is activated in condyloma acuminata,which is correlated with the downregulation of apoptosis,thus they might be involved in the pathogenesis of CA.

Key words: Condylomata acuminata, Telomerase, Apoptosis