中华皮肤科杂志 ›› 2003, Vol. 36 ›› Issue (2): 97-99.

• 论著 • 上一篇    下一篇

胎盘中提取的黑素生成素有效成分分析及动物实验

许爱娥, 尉晓冬, 武家才, 沈宏, 洪为松   

  1. 杭州市第三人民医院 310009
  • 收稿日期:2002-01-04 出版日期:2003-02-15 发布日期:2003-02-15

Analysis of Active Principles of Melagenine Extracted From Human Placenta and Their Effect on Ep-perimental Animal

XU Ai-e, WEI Xiao-dong, WU Jia-cai, SHEN Hong, HONG Wei-song   

  1. Department of Dermatology, Third People's Hospital, Hangzhou 310009, China
  • Received:2002-01-04 Online:2003-02-15 Published:2003-02-15

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摘要： 目的 分析黑素生成素中的活性成分及其对豚鼠表皮中黑素细胞的影响。方法 利用放免法及比色法分别测定黑素生成素中内皮素和唾液酸的含量;用黑素生成素外搽豚鼠背部皮肤加红外线照射,5周后,对搽药的皮肤和对照皮肤进行活检,分别制备透射电镜超薄切片及不同的特殊染色的病理切片。统计搽药表皮病理切片中黑素细胞数目及黑素含量的变化并进行超微结构观察。结果 测定黑素生成素中内皮素含量为210.5±30.1pg/mL,唾液酸含量为147.9±12.1μg/mL。用黑素生成素外搽动物表皮后黑素细胞数目明显增加;含有黑素颗粒的角质形成细胞数、黑素含量指数(MCI-1、2)均有明显提高。结论 黑素生成素中含有能促进黑素细胞增殖的内皮素及增强细胞间识别与粘附的糖和糖脂类物质,作用于动物表皮具有促进黑素细胞增殖和黑素合成的功能。

关键词: 黑素类, 黑素细胞, 内皮缩血管肽, 唾液酸类

Abstract: Objective To analyze the active principle of Melagenine and its effect on melanocytes of guinea epidermis.Methods The content of endothelins and sialic acid of Melagenine were determined by radioimmunoassay and spectrophotometer.The skin of the back of guinea pigs was treated with Melagenine and irradiated with infraredray.Then the biopsy specimens were taken from the treated and untreated back skin,sections and supe-rthin sections were prepared for special staining and TEM examination,respectively,the number melanocytes and melanin content index(MCI)were measured.Results The contents of en-dothelins and sialic acid in melagenine were 210.5±30.1 pg/mL and 147.9±12.1μg/mL,respectively;the number of melanocytes,the keratinocytes with melanin granules and the melanin content index were all in-creased significantly in the treated skin.Conclusions There are endothelins,carbohydrates and glycolipids in melagenine,endothelins can promote the proliferation of melanocytes,while carbohydrates and glycolipids can enhance intercellular recognition and adhesion.The result of the study shows that melagenine promotes the proliferation of melanocytes and synthesis of melanin in the skinof guinea pigs.

Key words: Sialic acids, Melanins, Melanocytes, Endothelins