中华皮肤科杂志 ›› 2003, Vol. 36 ›› Issue (2): 88-90.

• 论著 • 上一篇    下一篇

维胺酯治疗痤疮的疗效观察及实验研究

李银涛1, 陈虹2, 李世荫3, 陈红清5, 黄卫宁4, 万苗坚1, 苏向阳1, 杨素莲1, 区凤仙1   

  1. 1. 中山大学附属第三医院皮肤科 广州 510630;
    2. 中山大学附属第三医院儿科 广州 510630;
    3. 北京大学附属第三医院皮肤科 广州 510630;
    4. 广州市皮肤病防治所;
    5. 广州医学院附属第一医院皮肤科 广州 510630
  • 收稿日期:2002-02-18 出版日期:2003-02-15 发布日期:2003-02-15

Clinical and In Vitro Study of Viaminate in Acne

LI Yin-tao1, CHEN Hong2, LI Shi-yin3, CHEN Hong-qing5, HUANG Wei-ning4, WAN Miao-jian1, SU Xiang-yang1, YANG Su-lian1, QU Feng-xian1   

  1. Department of Dermatology, Third Hospital, Zhongshan University, Guangzhou 510630, China
  • Received:2002-02-18 Online:2003-02-15 Published:2003-02-15

摘要: 目的 观察维胺酯治疗痤疮的临床疗效,并从分子水平上探讨维胺酯对雄激素受体的影响。方法 将痤疮患者分为观察组及对照组,观察组口服维胺酯4周,采用Samuelson九度分级法评定疗效;同时,体外培养角质形成细胞并用维胺酯处理,后用Northern杂交及Western杂交分别检测处理前后的该细胞雄激素受体的mRNA及蛋白质的表达水平。结果 观察组的总有效率为74.55%,显效率为44.55%,对照组总有效率为35.19%,显效率为23.15%,经卡方检验,两组总有效率及显效率差异均有显著性(P<0.0001);同时,体外培养的角质形成细胞用维胺酯处理后,其雄激素受体的mRNA的表达水平随维胺酯处理浓度的增加而呈逐渐下降趋势,其雄激素受体的蛋白质表达水平亦随维胺酯处理浓度的增加而呈逐渐下降趋势。结论 维胺酯不但临床上治疗痤疮疗效显著,而且实验结果提示其疗效与其下调角质形成细胞的雄激素受体有关。

关键词: 维甲酸, 痤疮, 寻常, 受体, 雄激素

Abstract: Objective To investigate the therapeutic effect of oral viaminate(a vitamine A acid derivative)on acne and its effect on the expression of androgen receptor.Methods Two hundred and eigh-teen patients with acne were allocated to treatment group and control group at random:treatment group was treated by oral viaminate;control group was treated by oral minocycline.The treatment lasted for four weeks.The effect of viaminate on the expression of androgen receptor was studied in COLO-16 cells in vitro by using Northern blot or Western blot.Results The therapeutic effect of oral viaminate in treatment group was higher than that in control group(P<0.0001).After treatment by viaminate,the expression of mRNA and proteins of the androgen receptors on COLO-16 cells was down-regulated,and was related to the concentration of viami-nate.Conclusion Viaminate has significant therapeutic effect on acne.In vitro study shows that the thera-peutic effect of viaminate on acne may be related to its down-regulation in the expression of androgen receptors on keratinocytes.

Key words: Vitamine A acid, Acne, vulgaris, Receptor, androgen