中华皮肤科杂志 ›› 2003, Vol. 36 ›› Issue (2): 100-102.

• 论著 • 上一篇    下一篇

尖锐湿疣病损组织粗提蛋白对树突状细胞免疫表型的影响

芦桂青1, 程浩1, 吴金民2, 张在云3, 方向明3, 王琦3, 赵可佳1   

  1. 1. 杭州熏浙江大学医学院附属邵逸夫医院皮肤科 310016;
    2. 杭州熏浙江大学医学院附属邵逸夫医院肿瘤中心 310016;
    3. 浙江大学医学院邵逸夫临床研究所 310016
  • 收稿日期:2002-01-15 出版日期:2003-02-15 发布日期:2003-02-15
  • 基金资助:
    国家教育部基金(491030-G50113)

Effects on Immunophenotypes of Dendritic Cells Induced by Crude Extracts from Lesions of Condyloma Acuminata

LU Gui-qing1, CHENG Hao1, WU Jin-min2, ZHANG Zai-yun3, FANG Xiang-ming3, WANG Qi3, ZHAO Ke-jia1   

  1. Department of Dermatology, Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, Hangzhou 310016, China
  • Received:2002-01-15 Online:2003-02-15 Published:2003-02-15

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摘要： 目的 研究尖锐湿疣病损组织粗提蛋白对树突状细胞免疫表型的影响。方法 贴壁法分离脐血和外周血中的单核细胞,流式细胞仪检测树突状细胞特异性表面分子的表达。结果 rhGM-CSF、rhIL-4和LPS可诱导脐血和外周血来源的单核细胞分化为成熟的树突状细胞。尖锐湿疣病损粗提蛋白刺激后的树突状细胞高表达CD86穴脐血91.7%熏外周血88.6%雪和HLA-DR穴脐血88.4%熏外周血85.0%雪熏而包皮刺激组的CD86和HLA-DR的表达较低穴CD86押脐血87.1%熏外周血77.2%;HLA-DR押脐血68.0%熏外周血59.7%雪,空白对照组的CD86表达更低穴脐血83.5%熏外周血62.4%雪,HLA-DR的表达介于前两者之间(脐血72.7%熏外周血68.3%)。结论 尖锐湿疣病损粗提蛋白使树突状细胞部分抗原递呈相关分子表达呈上调趋势,提示尖锐湿疣粗提蛋白可能增强树突状细胞的抗原递呈功能并进而促进T细胞的激活。

关键词: 尖锐湿疣, 树突细胞, 免疫表型分型

Abstract: Objective To investigate the effects of crude extracted proteins from lesions of condyloma acuminata on immunophenotypes of dendritic cells.Methods Plastic-adherent mononuclear cells(MNCs)were isolated from umbilical cord blood or peripheral blood and cultured in media containing cytokines(GM-CSF,IL-4and LPS).The morphology and phenotypes of these cells were analyzed by flow cytometry and microscopy in 12 day's culture.Cells on the fourth day were incubated with crude extracts from lesions of condyloma acuminata,foreskin proteins,and PBS,respectively,followed by phenotypic analysis after 9-12 days' culture.Results Expression of antigens CD1a,CD80,CD86,MHC-I,MHC-II,CD14,CD54 was detected after 12 days' culture.It was shown that MNCs could be induced to differentiate to mature dendritic cells in our culture system.After incubation with crude extracts from lesions of condyloma acuminata for another 9-12 days,expression of CD86 and HLA-DR was increased on dendritic cells.Conclusions Compared to foreskin and PBS pulsed dendritic cells,expression of CD86 and HLA-DR is upregulated on dentritic cells after pulsing with condyloma acuminata lesion proteins.The data suggest that crude extracts from lesions of condyloma acuminata might enhance antigen-presenting capacity of dendritic cells and strengthen activation of T lymphocytes.

Key words: Condylomata acuminata, Dendritic cells, Immunophenotyping