中华皮肤科杂志 ›› 2003, Vol. 36 ›› Issue (12): 699-701.

• 论著 • 上一篇    下一篇

HSPC011基因在毛乳头细胞的表达及其cDNA克隆

杨卫兵, 郝飞, 宋志强, 杨希川   

  1. 第三军医大学西南医院皮肤科 重庆 400038
  • 收稿日期:2002-12-02 出版日期:2003-12-15 发布日期:2003-12-15
  • 通讯作者: 郝飞,E-mail:haofei@mail.tmmu.com.cn E-mail:haofei@mail.tmmu.com.cn
  • 基金资助:
    国家自然科学基金(30200249)

The Expression of Gene HSPC011 in Dermal Papilla Cells of Hair Follicle and Its cDNA Cloning

YANG Wei-bing, HAO Fei, SONG Zhi-qiang, YANG Xi-chuan   

  1. Department of Dermatology, Southwest Hospital, Third Military Medical University, Chongqing 400038, China
  • Received:2002-12-02 Online:2003-12-15 Published:2003-12-15

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摘要： 目的 确定HSPC011基因在毛乳头细胞的表达,并克隆其cDNA.方法 原位杂交技术证实HSPC011基因在毛乳头细胞表达;RT-PCR扩增目的基因并构建重组真核表达质粒pCI-neo/HSPCO11.结果 细胞内mRNA原位杂交证实HSPC011基因在凝集性生长的毛乳头细胞表达,而在非凝集性生长的毛乳头细胞和人真皮成纤维细胞不表达;RT-PCR从毛乳头细胞RNA中扩增到约430bp目的基因片段;构建了重组质粒pCI-neo/HSPCO11,酶切分析和测序结果均表明目的片段与HSPC011基因相应序列完全一致.结论 HSPC011基因在毛乳头细胞表达,其表达可能与毛乳头细胞的分化和功能状态有关.

关键词: 毛囊, 基因表达, 造血干细胞

Abstract: Objective To identify the expression of gene HSPC011(HSPC,hematopoietic stem/progenitor cell)in the dermal papilla cells of hair follicle and clone its cDNA.Methods The expression of gene HSPC011 was confirmed by intracellular mRNA hybridization in situ;the objective cDNA was amplified by RT-PCR(reverse transcription PCR).Results By in situ hybridization,it was shown that gene HSPC011expressed in the coagulated dermal papilla cells,but not in the non-coagulated dermal papilla cells and the dermal fibroblast;The objective cDNA with a fragment of 430bp was amplified by RT-PCR,and a recombinant eukaryotic expressing plasmid pCI-neo/HSPC011 was constructed.By enzyme cutting and sequencing analysis,the objective cDNA was completely identical with gene HSPC011.Conclusion Gene HSPC011 was clearly shown to express in the dermal papilla cells,and the expression of HSPC011 was possibly related with the differentiation and functional status of the dermal papilla cells.

Key words: Hair follicle, Gene expression, Hematopoietic stem cells