中华皮肤科杂志 ›› 2003, Vol. 36 ›› Issue (12): 682-684.

• 论著 • 上一篇    下一篇

红色毛癣菌基因型与表型的研究

杨国玲1, 李乔1, 于晓虹1, 刘维达2, 林熙然1   

  1. 1. 大连医科大学附属第一医院皮肤科 116011;
    2. 中国医学科学院、中国协和医科大学皮肤病研究所
  • 收稿日期:2002-12-31 出版日期:2003-12-15 发布日期:2003-12-15

Study on the Relationship Between Genotypes and Phenotypes of Trichophyton rubrum

YANG Guo-ling1, LI Qiao1, YU Xiao-hong1, LIU Wei-da2, LIN Xi-ran1   

  1. Department of Dermatology, First Affiliated Hospital, Dalian Medical University, Dalian 116011, China
  • Received:2002-12-31 Online:2003-12-15 Published:2003-12-15

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摘要： 目的 探讨红色毛癣菌基因型与表型、侵犯部位及其来源地区的相关性.方法 基因分型采用ITS区域探针与DNA印迹杂交法,表型分型采用传统方法.结果 所试49株红色毛癣菌(南京21株、大连26株、北京2株)分为20型(A-T型),其中A型9株均为大连株;B型9株中7株为南京株;C型6株均为南京株.5种表型除颗粒型外其他4型均有A型,17株绒毛型和7株沟纹型B型占居首位,6株羊毛型和17株粉末型A型占居首位.分离自甲癣的21株以C型为主;股癣10株和体癣6株以A型为主;足癣8株,B型占居首位;头癣4株G型占居首位.结论 红色毛癣菌基因型与表型、侵犯部位及其来源地区可能具有一定的相关性.

关键词: 发癣菌属, 基因型, 表型

Abstract: Objective To study the relationship between genotypes and phenotypes,geographical distribution,and the sites of infection of Trichophyton rubrum(T.rubrum).Methods The genotypes were determined by Southern blotting with a probe amplified from the small-subunit rDNA and adjacent internal transcribed spacer(ITS)regions.The phenotypes of T.rubrum were determined by conventional method.Results Twenty genotypes(DNA type A to T)and 5 phenotypes(villous,furrowed,granular,powdery,and woolly)were recognized among 49 strains of T.rubrum.Genotype A prevailed in all phenotypes except granular type.Type B represented the most common genotype among the strains of villous type and furrowed type.Type A took the first place in powdery type and woolly type.All of the type A strains were from Dalian.Seven of 9 type B strains were from Nanjing.Six type C strains were all from Nanjing.The majority strains of 21 strains isolated from tinea unguium were type C,most of the16strains isolated from tinea cruris and tinea corporis were type A,8 strains from tinea pedis were type B,and4strains from tinea capitis were type C.Conclusion There are certain possible relationships between genotypes and phenotypes,geographical distribution and sites of infection of T.rubrum.

Key words: Trichophyton, Genotype, Phenotype