中华皮肤科杂志 ›› 2003, Vol. 36 ›› Issue (10): 589-590.

• 论著 • 上一篇    下一篇

端粒酶在瘢痕疙瘩及其周围外观正常皮肤中的表达

王强, 陈晓栋, 王焱, 张国成   

  1. 中国医学科学院、中国协和医科大学皮肤病研究所 南京 210042
  • 收稿日期:2002-09-18 出版日期:2003-10-15 发布日期:2003-10-15

Expression of Telomerase in Keloid and Adjacent Normal Skins

WANG Qiang, CHEN Xiao-dong, WANG Yan, ZHANG Guo-cheng   

  1. Insititute of Dermatology, Chinese Academy of Medical Science, Peking Union Medical College, Nanjing 210042, China
  • Received:2002-09-18 Online:2003-10-15 Published:2003-10-15

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摘要： 目的 探讨端粒酶的活性与瘢痕疙瘩发病的关系。方法 采用链霉亲和素-过氧化物酶(SP)免疫组化法,对17例瘢痕疙瘩标本、10例瘢痕疙瘩周围外观正常皮肤标本及9例正常人对照组标本的成纤维细胞中端粒酶的活性进行检测,用SPSS统计软件进行相应统计学分析。结果 在瘢痕疙瘩标本中,50.5%的成纤维细胞中端粒酶活性检出阳性,且阳性细胞平均染色较深;在瘢痕疙瘩周围外观正常皮肤标本中,21.1%的成纤维细胞中端粒酶活性检出阳性;在正常人皮肤标本中,7.9%的成纤维细胞中端粒酶活性检出阳性,且阳性细胞平均染色深度明显浅于前两组。各组间两两比较,差异均有显著性(P<0.05)。结论 端粒酶的活性增高在瘢痕疙瘩的发病机制中有重要作用。

关键词: 瘢痕疙瘩, 端粒, 末端转移酶

Abstract: Objective To investigate the role of telomerase in the pathogenesis of the keloid. Methods Expression of telomerase in 17 specimens from keloidal lesions, 10 specimens from normal skin adjacent to keloids and 9 skin specimens from normal controls were detected by immunohistochemistry tech-niques. Results It was shown that 50.5% of fibroblasts were positive (with telomerase activity) in keloids, 21.1% of fibroblasts positive in the normal skin adjacent to keloids, and only 7.9% of fibroblasts positive in the skin of normal controls, and expressing less degree of staining in the positive fibroblasts than that in keloids and its adjacent normal skins. Consequently, the expression of telomerase was significantly higher in both keloid and its adjacent normal skins than that in normal controls. Conclusion High level of telomerase expression may be related to the pathogenesis of the keloid.

Key words: Keloid, Telomerase