中华皮肤科杂志 ›› 2003, Vol. 36 ›› Issue (10): 586-588.

• 论著 • 上一篇    下一篇

甘草黄酮对黑素细胞生物学作用的比较

马晶波, 黄岚, 冯树芳, 郑志忠, 祝禄川   

  1. 复旦大学附属华山医院皮肤科 上海 200040
  • 收稿日期:2002-09-20 出版日期:2003-10-15 发布日期:2003-10-15

Biological Effects of Glabridin on Melanocytes

MA Jing-bo, HUANG Lan, FENG Shu-fang, ZHENGZhi-zhong, ZHU Lu-chuan   

  1. Department of Dermatology, Huashan Hospital, Fudan University, Shanghai 200040, China
  • Received:2002-09-20 Online:2003-10-15 Published:2003-10-15

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摘要： 目的 研究甘草黄酮对体外培养的鼠黑素瘤B16细胞及人表皮黑素细胞的生物学作用。方法 建立正常人表皮黑素细胞培养体系和培养黑素瘤B16细胞,添加甘草黄酮后,测定黑素细胞活力、酪氨酸酶活性以及黑素含量的变化,并与氢醌的作用结果进行比较。结果 两种黑素细胞对药物的反应有所不同。与氢醌比较,甘草黄酮具有较强的浓度依赖性黑素合成抑制作用,且对黑素细胞的细胞毒性较小。结论 用体外培养的人黑素细胞作为研究对象与鼠黑素瘤B16细胞比较具有一定的差异;甘草黄酮可作为一种安全有效的皮肤美白物质。

关键词: 黑素细胞, 黑素类, 一元酚单氧酶, 甘草黄酮

Abstract: Objective To study the effects of glabridin on human melanocytes and B16 murine melanoma cells. Methods After glabridin was added into the two kinds of cultured melanocytes, the cell viability, tyrosinase activity and melanin contents were measured, respectively. The effects of glabridin were compared with those of hydroquinone. Results It was shown that the effects of glabridin and hydroquinone on two kinds of cells were different. Compared with hydroquinone, glabridin had a concentration-dependent inhibition on melanogenesis and little influence on melanocyte viability. Conclusion There is a biological diversity between human melanocytes and murine melanoma cells. It is indicated that glabridin is a safe and active ingredient for depigmentation.

Key words: Melanocytes, Melanin, Monophenol monooxygenase, Glabridin