中华皮肤科杂志 ›› 2003, Vol. 36 ›› Issue (1): 28-30.

• 论著 • 上一篇    下一篇

胚胎胸腺移植治疗BXSB小鼠自发性系统性红斑狼疮的研究

付继成, 徐世正, 周心房, 熊腊元, 张岚, 郑卫   

  1. 武汉大学人民医院皮肤科, 430060
  • 收稿日期:2002-01-19 出版日期:2003-01-15 发布日期:2003-01-15

Embryo Thymus Transplantation in the Treatment of Lupus-Like BXSB Mice

FU Ji-cheng, XU Shi-zheng, ZHOU Xin-fang, XIONG La-yuan, ZHANG Lan, ZHENG Wei   

  1. Department of Dermatology, Renmin Hospital, Wuhan University, Wuhan 430060, China
  • Received:2002-01-19 Online:2003-01-15 Published:2003-01-15

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摘要： 目的 通过胚胎胸腺移植治疗BXSB小鼠自发性系统性红斑狼疮(SLE),了解SLE的发病机制以及胚胎胸腺移植的临床应用前景。方法 将正常胚胎小鼠胸腺移植于50d大小的雄性BXSB小鼠体内,定期检测小鼠尿蛋白、血清抗核抗体、血尿素氮、血肌酐,肾小球免疫球蛋白沉着情况及因SLE而死亡的情况,连续观测5个月。结果 移植组5、6、7月龄小鼠尿蛋白、血抗核抗体、血尿素氮及肌酐均明显低于5月龄对照组小鼠;地塞米松治疗组结果与移植组小鼠相似;移植组及地塞米松治疗组SLE的死亡率明显减低;移植后和地塞米松治疗后各月龄小鼠肾小球免疫球蛋白沉着阳性率与对照组差异无显著性。结论 胚胎胸腺移植能改善BXSB小鼠肾功能,降低血抗核抗体滴度,延长小鼠的寿命,疗效与地塞米松治疗类似,胚胎胸腺移植治疗BXSB小鼠自发性SLE有较好的近期疗效,胸腺的缺陷在BXSB小鼠的致病中起重要作用,胚胎胸腺移植有望应用于临床SLE的治疗。

关键词: 胎组织移植, 红斑狼疮,系统性, 动物,实验

Abstract: Objective To evaluate the efficacy of embryo thymus transplantation in the treatment of lupus-like BXSB mice,study the pathogenesis of SLE in BXSB mice and the therapeutic effect of embryo thymus transplantation.Methods The embryonic thymus of CB57L mice was transplanted to 50 day-old male BXSB mice.Levels of proteinuria,ANA,blood urea nitrogen(BUN),blood creatinine and the deposits of immunoglobulin(Ig)in the glomerulus were detected regularly for 5 months,and the number of mice died of SLE was observed.Results The levels of proteinuria,ANA,BUN,blood creatinine of the 5,6,7 month old mice in embryo thymus transplantation group were lower than that of 5 month old mice in control group.The efficacy of treatment in embryo thymus transplantation group were similar to that of dexamethasone treatment group,and the SLE-caused death was reduced in these two groups.However,the embryo thymus transplantation seemed not to reduce the deposits of lg in glomerulus significantly,the deposits of lg in the glomerulus were similar in all three groups.Conclusions Embryo thymus transplantation could improve renal functions and reduce the titer of ANA.Its efficacy is similar to that of dexamethasone.Embryo thymus transplantation has a short term therapeutic effect in the treatment of lupus-like BXSB mice.The deficiency of thymus in the BXSB mice may play an important role in the pathogenesis of lupus-like mice.Embryo thymus transplantation may be a valuable approach to treat SLE.

Key words: Fetal tissue transplantation, Lupus erythematosus,systemic, Animals,experimental