中华皮肤科杂志 ›› 2002, Vol. 35 ›› Issue (5): 378-379.

• 论著 • 上一篇    下一篇

维生素D3抑制角质形成细胞CXCR2的表达

唐玲1, 于益芝2, 郭志丽1, 李泉1, 陶苏江1, 顾军1, 郑茂荣1   

  1. 1. 第二军医大学附属长海医院皮肤科, 上海200433;
    2. 第二军医大学免疫学研究所
  • 收稿日期:2001-09-05 出版日期:2002-10-15 发布日期:2002-10-15
  • 基金资助:
    国家自然科学基金(39970689);上海市科技启明星计划资助(99QB14047)

The Inhibitory Effect of Vit D3 on the Expression of CXCR2 on Keratinocytes

TANG Ling1, YU Yizhi2, GUO Zhili1, LI Quan1, TAO Sujiang1, GU Jun1, ZHENG Maorong1   

  1. Department of Dermatology, Changhai Hospital, The Second Military Medical University, Shanghai 200433, China
  • Received:2001-09-05 Online:2002-10-15 Published:2002-10-15

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摘要： 目的 观察维生素D3对角质形成细胞表面表达CXCR2的影响,探讨其在治疗银屑病中的作用机制。方法 用不同浓度的维生素D3处理人角质形成细胞株HaCaT,48h后通过四甲基偶氮唑盐(MTT)法检测了维生素D3抑制HaCaT增殖的有效剂量;通过流式细胞仪(FACS)分析维生素D3处理的HaCaT表达趋化因子受体CXCR2的情况。结果 HaCaT经维生素D3在8.0×10-8mol/L～5.12×10-6mol/L浓度范围内处理48h后的A值较未处理组明显降低(P<0.05).HaCaT在5.12×10-6mol/L维生素D3处理48h后CXCR2的表达明显低于正常对照组。结论 维生素D3治疗银屑病的作用机制部分是通过抑制角质形成细胞表达CXCR2,从而达到抑制角质形成细胞增殖的目的。

关键词: 胆骨化醇, 角蛋白细胞, CXCR2

Abstract: Objective To detect the expression of CXCR2 on psoriatic lesional keratinocytes and to in-vestigate the mechanism of Vit D3 in the treatment of psoriasis.Methods The proliferative capacity of human keratinocyte cell line,HaCaT,treated with Vit D3 was detected by MTT assays.The expression of CXCR2 on HaCaT treated with Vit D3 was assayed by FACS calibur.Results The Vit D3 at proper concentrations could inhibit the proliferation of HaCaT and down-regulate the CXCR2 expression on the cells.Conclusion One of the mechanisms for Vit D3 in the treatment of psoriasis might be the inhibition of CXCR2 expression on keratinocytes,which con-tributes to inhibit the over-proliferation of keratinocytes in psoriasis.

Key words: Cholecalciferol, Keratinocytes, CXCR2