中华皮肤科杂志 ›› 2002, Vol. 35 ›› Issue (5): 346-348.

• 论著 • 上一篇    下一篇

白念珠菌性阴道炎动物模型的构建

赵敬军, 沈永年, 陈伟, 刘维达   

  1. 中国医学科学院、中国协和医科大学皮肤病研究所, 南京210042
  • 收稿日期:2002-01-11 出版日期:2002-10-15 发布日期:2002-10-15

A Murine Model of Candida albicans Vaginitis

ZHAO Jingjun, SHEN Yongnian, CHEN Wei, LIU Weida   

  1. Institute of Dermatology, Chinese Academy of Medical Sciences and Peking Union Medical College, Nanjing 210042, China
  • Received:2002-01-11 Online:2002-10-15 Published:2002-10-15

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摘要： 目的 构建白念珠菌性阴道炎的小鼠模型。方法 采用ICR雌性小鼠,预先给其皮下注射苯甲酸雌二醇以造成小鼠的假发情,再给予小鼠阴道内注射约5×104个白念珠菌稳态芽生孢子,动态观察其阴道分泌物生成及镜检情况;并进行阴道灌洗液的真菌载量分析以及阴道组织病理观察。结果 与未雌激素化的小鼠比较,雌激素化小鼠阴道灌洗液的真菌载量分析显示自第2天开始可分离出高水平的计数结果并直到接种后21d;阴道组织病理结果发现自第4天至实验结束均可见到阴道粘膜浅层有菌丝生长,并有炎性细胞浸润。结论 此动物模型可以作为一个研究念珠菌性阴道炎发病机制的手段。

关键词: 念珠菌,白色, 念珠菌病,外阴阴道, 疾病模型,动物

Abstract: Objective To construct a murine model of vaginal candidiasis.Methods A kind of pseu-doestrus(estrogen-treated)mice was inoculated with 5×104 stationary-phase blastoconidia of Candida albicans in-travaginally,and the vaginal lavage fluid and vaginal tissues were obtained at day 2,4,7,14 and 21 after inoculation.Results Compared with non-estrogen-treated mice,there was high load of C.albicans in the culture of lavage fluid from the second day to the twenty-firstt day after inoculation;Vaginal tiss sue from C.albicans-inoculated mice contained large amount of hyphae in the superficial mucosa.Conclusion This animal model of C.albicans vaginitis provides a means to study the pathogenesis of mucosal C.albicans infections.

Key words: Candida albicans, Candidiasis,vulvovaginal, Disea se models,animal