中华皮肤科杂志 ›› 2002, Vol. 35 ›› Issue (4): 296-298.

• 论著 • 上一篇    下一篇

卡泊三醇、维A酸及雷公藤内酯醇对角质形成细胞及COLO16细胞中血管内皮生长因子生成的影响

周海燕, 郑家润, 李新宇, 陈沄   

  1. 中国医学科学院、中国协和医科大学皮肤病研究所 南京 210042
  • 收稿日期:2001-11-27 出版日期:2002-08-15 发布日期:2002-08-15
  • 基金资助:
    国家高校博士点基金资助(200011)

Effects of Calcipotriene,Tretinoin and Triptolide on Production of Vascular Endothelial Growth Factor in Keratinocytes and COLO16 Cell Line

ZHOU Haiyan, ZHENG Jiarun, LI Xinyu, CHEN Yun   

  1. Institute of Dermatology, Chinese Academy of Medical Sciences & Peking Union Medical College, Nanjing 210042, China
  • Received:2001-11-27 Online:2002-08-15 Published:2002-08-15

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摘要： 目的 探讨卡泊三醇、维A酸及雷公藤内酯醇对角质形成细胞及COLO16细胞中血管内皮生长因子(VEGF)生成的影响.方法 采用RT-PCR法检测原代培养的人角质形成细胞及COLO16细胞中VEGFmRNA的水平.结果 卡泊三醇作用于正常人角质形成细胞4h和24h后,以及作用COLO16细胞24h后,均可抑制VEGFmRNA的表达,呈剂量依赖性,IC50值分别为1.19×10-4μg/mL、1.51×10-4μg/mL和3.16×10-5μg/mL.维A酸作用于正常人角质形成细胞4h后可抑制VEGFmRNA的表达,作用于正常角质形成细胞24h后及作用于COLO16细胞4h和24h均无抑制作用.雷公藤内酯醇对正常角质形成细胞和COLO16细胞中VEGFmRNA表达均无抑制作用.结论 在转录水平抑制VEGF生成可能是维A酸与卡泊三醇抗银屑病的作用机制之一.

关键词: 内皮生长因子, 卡泊三醇, 维甲酸, 雷公藤内酯醇

Abstract: Objective To investigate the effects of calcip otriene,tretinoin and triptolide on mRNA expression of vascular endothelial growth factor(VEGF)in primary cultured keratinocytes a nd COLO16 cell line.Methods VEGF mRNA levels were determined by reverse transcriptase polymerase ch ain reaction(RT-PCR).Results Calcipotriene inhibited the mRNA expression of VEGF in a dose-dependent manner in keratinocytes after4hour and 24hour treatment.The 50%i nhibitory concentation values(IC 50)of calcipotriene were 1.19×10-4μg/mL(KC 4h),1.51×10-4μg/mL(KC 24h)and 3.16×10-5μg/mL(COLO1624h)respectively.Tretinoin in-hibited the mRNA expression of VEGF in keratinocytes after 4hours,and had no inhibition in keratinocytes after 24hours and in COLO16 cells after 4hour s and 24hours.Triptolide had no inhibition.Conclusions The antipsoriatic effect of calcipotriene and tretinoin may be partly explained by their in hibition on VEGF production at transcription level.

Key words: Endothelial growth factors, Calcipotriene, Tretinoin, Triptolide