中华皮肤科杂志 ›› 2002, Vol. 35 ›› Issue (4): 284-286.

• 论著 • 上一篇    下一篇

蕈样肉芽肿的端粒酶研究

应作霖1, 孙建方1, 张行2, 姜祎群1, 许则峰2, 曾学思1, 桑红桂1   

  1. 1. 中国医学科学院、中国协和医科大学皮肤病研究所;
    2. 浙江大学肿瘤研究所
  • 收稿日期:2001-08-13 出版日期:2002-08-15 发布日期:2002-08-15
  • 通讯作者: 孙建方,中国医学科学院、中国协和医科大学皮肤病研究所,南京,210042

Detection of Telomerase Activity in Patients with Mycosis Fungoides

YING Zuolin1, ZHANG Hang1, JIANG Yiqun2, XU Zefeng1, ZENG Xuesi2, SANG Honggui1   

  1. Institute of Dermatology, Chinese Academy of Medical Sciences & Peking Union Medical College, Nanjing 210042, China
  • Received:2001-08-13 Online:2002-08-15 Published:2002-08-15

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摘要： 目的 检测各期蕈样肉芽肿(MF)的端粒酶活性,探讨端粒酶在MF肿瘤发生机制中的作用.方法 采用端粒酶聚合酶链反应-酶联免疫吸附测定法(PCR-ELISA),对35例MF患者进行端粒酶的定性和定量分析.结果 92.3%的肿瘤期MF、78.6%的斑块期MF和75.0%的斑片期MF显示端粒酶阳性;肿瘤期MF的端粒酶活性水平高于斑块期和斑片期,且差异有显着性;而斑块期与斑片期之间差异无显着性.对照组的端粒酶均为阴性.讨论 MF患者存在高水平的端粒酶活性,提示端粒酶可能在MF的肿瘤发生中起重要作用,对MF的诊断有一定的临床意义.

关键词: 蕈样肉芽肿, 端粒酶

Abstract: Objective To detect telomerase activity in patients with mycosis fungoides(MF)and study the role of telomerase in the tumorigenesis of MF.Methods Polymerase chain reaction-enzyme linked immunosorbent assay(PCR-ELISA)was employed to detect telomerase activity in 35 patients with different stages of mycosis fungoides.Results Telomerase was positively detected in 92.3% patients with tumor stage,78.6% plaque stage and 75.0% patch stage of MF.Telomera se activity was significantly higher in tumor stage than that in plaque and patch stage.No significant differe nce of telomerase activity was found between plaque stage and patch stage.No telomerase activity was detected in control group.Conclusions High level of telomerase activity is observed in MF patients,suggesting that telomerase may play an important role in the tumorigenesis of MF and may serve as a useful marker for the diagnosis of MF.

Key words: Mycosis fungoides, Telomerase