中华皮肤科杂志 ›› 2002, Vol. 35 ›› Issue (4): 269-271.

• 论著 • 上一篇    下一篇

EB病毒感染与系统性红斑狼疮相关性的研究

谭国珍, 曾凡钦, 陈宜芳, 李伯有, 李希清   

  1. 中山大学附属第二医院 广州 510120
  • 收稿日期:2001-07-02 出版日期:2002-08-15 发布日期:2002-08-15

The Correlation Between Epstein-Barr Virus Infection in B Lymphocytes and Systemic Lupus Erythematosus

TAN Guozhen, ZENG Fanqin, CHEN Yifang, LI Boyou, Li Xiqing   

  1. Department of Dermatology, Sun Yat-sen Memorial Hospital, Sun Yat-sen University of Medical Sciences, Guangzhou 510120, China
  • Received:2001-07-02 Online:2002-08-15 Published:2002-08-15

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摘要： 目的 探讨EB病毒感染与系统性红斑狼疮发生、发展的关系.方法 用流式细胞仪双色解析法检测SLE患者外周血CD19+、CD23+B淋巴细胞中EB病毒潜伏膜蛋白LMP1和复制期蛋白ZEBRA的表达.结果 活动期患者CD19+细胞和CD23+细胞LMP1的表达率均高于稳定期患者,两组患者均高于对照组,且患者CD23+细胞LMP1的表达率高于CD19+细胞,而对照组两者差异无显着性;ZE-BRA的表达仅见于SLE患者外周血B淋巴细胞中,活动期患者CD19+、CD23+细胞ZEBRA的表达率均高于稳定期患者,且在活动期患者中,CD23+细胞ZEBRA的表达率高于CD19+细胞,而在稳定期患者中,CD23+细胞和CD19+细胞ZEBRA的表达率差异无显着性.结论 EB病毒的感染和复制与B淋巴细胞的活化及SLE的发生和发展有关.

关键词: 红斑狼疮,系统性, B淋巴细胞, 疱疹病毒4型,人

Abstract: Objective To investigate the correlation between Epstein-Barr virus(EBV)infection and the initiation and development of systemic lupus erythematosus.Methods The expression of EBV-LMP1 and E-BV-ZEBRA was detected by two-colour cytofluorography in CD19+、CD23+ B lymphocytes in peripheral blood of SLE patients.Results The expression of EBV-LMP1 in CD19+ Bcells and CD23+ Bcells was significantly increased in active stage patients compared with that in remission stage patients,an d in patient group compared with that in controls.The frequencies of expression of EBV-LMP1were significantly higher in CD23+ B cells than that in CD19+ Bcells in SLEpatients,but not in normal controls.The expression of EBV-ZEBRA could be detected in SLE patients only,and its expression in CD19+B cells and CD23+ B cells was significantly higher in a ctive stage than that in remission stage.The frequencies of expression of EBV-ZEBRA were significantly higher in CD23+ Bcells than that in CD19+ B cells in active stage,but not in remission stage.Conclusion EBV infection and replication lead to overactivation of B lymphocytes,which might be related to the initiation and development of SLE.

Key words: Lupus erythematosus,systemic, B-lymphocytes, Herpesvirus 4,human