中华皮肤科杂志 ›› 2002, Vol. 35 ›› Issue (4): 256-259.

• 论著 • 上一篇    下一篇

CTLA4-Ig对变应性接触性皮炎小鼠模型的治疗作用

周倩1, 寇庚1, 钱卫珠1, 王皓1, 刘彦君1, 王晓芸1, 宋水川1, 金军1, 李晓冬1, 吴孟超2, 郭亚军1, 陈列平3   

  1. 1. 第二军医大学国际合作肿瘤研究所 上海 200433;
    2. 第二军医大学东方肝胆外科研究所;
    3. 美国莫尔医学院免疫学系
  • 收稿日期:2001-09-07 出版日期:2002-08-15 发布日期:2002-08-15
  • 基金资助:
    国家自然科学基金资助(39928012)

Treatment of Murine Allergic Contact Dermatitis with CTLA4-Ig

ZHOU Qian1, KOU Geng1, QIAN Weizhu1, WANG Hao1, LIU Yanjun1, WANG Xiaoyun1, SONG Shuichuan1, JIN Jun1, LI Xiaodong1, WU Mengchao2, GUO Yajun1, CHEN Lieping3   

  1. International Joint Cancer Institute, Second Military Medical University, Shanghai 200433, China
  • Received:2001-09-07 Online:2002-08-15 Published:2002-08-15

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摘要： 目的 探讨B7/CD28共刺激通路阻断剂CTLA4-Ig对变应性接触性皮炎的作用.方法 建立小鼠二硝基氟苯(DNFB)变应性接触性皮炎模型,以不同剂量的CTLA4-Ig进行静脉注射,通过检测小鼠耳廓肿胀度观察其对DNFB和FITC刺激的反应,并观察CTLA4-Ig的长期疗效,同时对治疗后的小鼠脾细胞进行体外淋巴细胞增殖实验.结果 CTLA4-Ig有明显的治疗作用,DNFB变应性接触性皮炎小鼠经治疗耳廓肿胀抑制率最高达69.7%,而且治疗的小鼠在激发后的14d用DNFB再刺激能产生无应答反应,但对FITC的再刺激表现为耳廓肿胀明显,过继转移CTLA4-Ig治疗小鼠的淋巴细胞能诱导受体鼠对DNFB的刺激产生特异性免疫耐受.结论 应用B7/CD28共刺激通路阻断剂CTLA4-Ig融合蛋白能有效地抑制接触性超敏反应,为临床治疗Ⅳ型变态反应性皮肤病如湿疹等提供一种新的可能的治疗策略.

关键词: 皮炎,变应性接触性, CTLA4-Ig

Abstract: Objective To study the effects of CTLA4-Ig on murine allergic contact dermatitis.Methods Mice were exposed to DNFB to induce allergic contact dermatitis and were injected with CTLA4-Ig.Ear swelling was measured 24h after antigen challenge.Splenocytes from treated mice were assayed for their ability to prolif erate in response to DNFB or FITC stimulation in vitro.Results Profound inhibition of contact hypersensitivity response(CHS) was shown by 69.7% in mice treated with CTLA4-Ig compared with mice treated with PBS control.CT-LA4-Ig-treated mice displayed DNFB-specific tolerance,but exhibited a vigorous immune response to FITC when re-sensitizing 14 days after the first challenge.Adoptive transfer of l ymphocytes from CTLA4-Ig-treated mice could induce inhibition of CHS in recipient mice.Conclusions CTLA4-Ig can inhibit CHS by blocking B7/CD28 co-stimulatory pathway,which provides a new way to suppress typeⅣ allergic reaction.

Key words: Dermatitis,allergic contact, CTLA4-Ig