p63、增殖细胞核抗原和表皮生长因子受体在皮肤鳞状细胞癌中的表达

中华皮肤科杂志 ›› 2002, Vol. 35 ›› Issue (2): 143-145.

p63、增殖细胞核抗原和表皮生长因子受体在皮肤鳞状细胞癌中的表达

王建力¹, 陈莉², 王桂兰², 曹晓蕾²

1. 南通医学院附属医院皮肤科 226001;
2. 南通医学院病理教研室 226001

收稿日期:2001-08-22 出版日期:2002-04-15 发布日期:2002-04-15
基金资助:
江苏省科委资助(Bs99358)

Expression of p63、PCNA and EGFr in Skin Squamous Cell Carcinoma

WANG Jianli¹, CHEN Li², WANG Guilan², CHAO Xiaolei²

Department of Dermatology, Affiliated Hospital, Nantong Medical College, Nantong 226001, China

Received:2001-08-22 Online:2002-04-15 Published:2002-04-15

摘要/Abstract

摘要： 目的探讨皮肤鳞状细胞癌(SCC)中p63、增殖细胞核抗原(PCNA)和表皮生长因子受体(EGFr)的表达及其与癌增殖和分化的关系。方法采用免疫组化(LSAB)方法分析68例SCC中p63、PCNA和EGFr的表达。结果在正常和癌变上皮中p63、PCNA主要表达于具有增生潜能的基底细胞,而在终末分化的角质层细胞中缺如。高分化癌中p63呈环状分布于癌巢周围,低分化癌中p63阳性细胞增多,分布紊乱。癌中PCNA、EGFr表达于整个癌巢中。p63、PCNA、EGFr表达在SCC表皮内肿瘤(SIN)Ⅲ级中均明显高于正常或癌旁上皮,并在癌的不同分化中差异有显着性。在SCC中p63、PCNA、EGFr表达之间均有显着相关性。结论在SCC中由免疫组化显示p63、PCNA和EGFr过表达的癌细胞常是获得了更具增殖、侵袭和间变能力的细胞。

关键词: 癌,鳞状细胞, p63, 增殖细胞核抗原, 受体,表皮生长因子-尿抑胃素

Abstract: Objective To investigate expression of p63、PCNA and epidermal growth factor receptor(EGFr)in skin squamous cell carcinoma(SCC).Method Tissue specimens from 68 cases of SCC were studied using immunohistochemistry staining method with monoclonal antibodies to p63、PCNA and EGFr. Results The expression of p63 protein and PCNA was principally restricted to basal cells with high proliferative potentiality and was absent in cells of stratum corneum that were undergoing terminal differentiation in normal or carcinoma epidermis. In highly differentiated tumors, the staining of p63 formed a ring surrounding carcinoma nests. In low differentiated tumors, p63 positive cells were increased and disorderly arranged. Diffuse expression of PCNA and EGFr was found through out all carcinoma nests. Expression of p63、PCNA and EGFr was significantly stronger in SCC and intraepithelial neoplasm(SIN)level Ⅲthan those in normal or proliferative epidermis near the carcinoma. A significant correlation was found between expression of p63,PCNA,EGFr and degree of differentiation of SCC,and between p63 and EGFr,p63 and PCNA,PCNA and EGFr, respectively. Conclusion Overexpression of p63、PCNA?EGFr may be contributed to acquisition of enhanced capability of proliferation, aggression and anaplasia in SCC.

Key words: Carcinoma,skin squamous cell, p63, Proliferating cell nuclear antigen, Receptors,epidermal growth factor urogastrone

王建力, 陈莉, 王桂兰, 曹晓蕾. p63、增殖细胞核抗原和表皮生长因子受体在皮肤鳞状细胞癌中的表达[J]. 中华皮肤科杂志, 2002, 35(2): 143-145.

WANG Jianli,CHEN Li,WANG Guilan,CHAO Xiaolei. Expression of p63、PCNA and EGFr in Skin Squamous Cell Carcinoma[J]. Chinese Journal of Dermatology, 2002, 35(2): 143-145.

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