中华皮肤科杂志 ›› 2001, Vol. 34 ›› Issue (6): 417-419.

• 论著 •    下一篇

新疆Kaposi肉瘤组织内人类8型疱疹病毒DNA的检测

康晓静1, 沈大为2, 普雄明1, 吴卫东1, 周炳华1   

  1. 1. 新疆维吾尔自治区人民医院皮肤科 乌鲁木齐 830001;
    2. 新疆医科大学第一附属医院皮肤科
  • 收稿日期:2001-02-06 出版日期:2001-12-15 发布日期:2001-12-15

Detection of Human Herpesvirus 8 in Kaposi's Sarcoma by Fluorescence in situ Polymerase Chain Reaction in Xinjiang

KANG Xiaojing1, SHEN Dawei2, PU Xiongming1   

  1. Department of Dermatology, The People's Hospital of Xinjiang Uygur Autonomous Region, Urumqi 830001, China
  • Received:2001-02-06 Online:2001-12-15 Published:2001-12-15

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摘要： 目的 了解Kaposi肉瘤组织内人类8型疱疹病毒(HHV-8)在Kaposi肉瘤发病中的作用。方法 应用荧光原位聚合酶链反应(PCR)方法对20例新疆Kaposi肉瘤组织进行HHV-8DNA检测,Kaposi肉瘤组织损害包括结节12份、斑块6份、斑片2份。同时和20份非Kaposi肉瘤组织,包括纤维瘤组织18份,血管瘤组织2份进行对照。结果 20例新疆Kaposi肉瘤组织中有17例检测到HHV-8DNA(阳性率为85%),而20例非Kaposi肉瘤组织HHV-8DNA均为阴性。两组阳性率经校正χ2检验,χ2=26.2,P<0.001.实验中HHV-8DNA片段主要集中于Kaposi肉瘤组织的梭形瘤细胞和血管内皮细胞。除此之外,在Kaposi肉瘤表皮角质形成细胞内也检测到HHV-8DNA片段,但DNA片段的荧光强度明显弱于梭形瘤细胞和血管内皮细胞中的荧光强度。结论 在新疆Kaposi肉瘤的发生中,HHV-8起着一定的作用,可能参与了Kaposi肉瘤的发病,但不是Kaposi肉瘤发生的唯一病因。

关键词: Herpesvirus,Kaposi sarcoma-associated, Sarcoma,Kaposi, 荧光原位聚合酶链反应

Abstract: Objective To investigate the localization of human herpesvirus (HHV-8) in lesions of Kaposi's Sarcoma (KS) and explore the role of HHV-8 in the pathogenesis of KS in Xinjiang. Methods HHV-8 DNA was detected by fluorescence in situ polymerase chain reaction. A total of 40 paraffin-embeded specimens were studied, including 20 KS lesions (12 nodular, 6 plaque and 2 patch lesions) and 20 non-KS lesions (18 dermatofibroma and 2 hemangioma). Results HHV-8 DNA was detected in 17 (85%) of 20 KS lesions and none in non-KS lesions (P<0.001). Positive signals for HHV-8 were demonstrated in the nuclei of spindle-shaped tumor cells and endothelial cells. In addition, HHV-8 DNA were also present in epidermal keratinocytes, however, the intensity of fluorescent signals was weaker than that in spindle-shaped tumor cells and endothelial cells. Conclusion The result of this study suggests that HHV-8 may play a role in the pathogenesis of KS.

Key words: Sarcoma,Kaposi, Herpesvirus,Kaposi sarcoma associated, Fluorescence in situ polymerase chain reaction