中华皮肤科杂志 ›› 2001, Vol. 34 ›› Issue (5): 346-348.

• 论著 • 上一篇    下一篇

16种支孢随机扩增DNA多态性研究

席丽艳1, 龙泽香代子2, Kaoru Okada2, 吴绍熙3, 福岛和贵2   

  1. 1. 中山医科大学孙逸仙纪念医院皮肤科;
    2. 日本千叶大学真菌医学研究中心;
    3. 中国医学科学院、中国协和医科大学皮肤病研究所
  • 收稿日期:2000-09-03 出版日期:2001-10-15 发布日期:2001-10-15

Characterization of 16Cladosporium Species by Random Amplification of Polymorphic DNA Assay

XI Liyan1, Takizawa K2, Kaoru Okada2   

  1. Department of Dermatology, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University of Medical Sciences, Guangzhou 510120, China
  • Received:2000-09-03 Online:2001-10-15 Published:2001-10-15

PDF (PC)

74

赞

摘要： 目的 了解支孢属腐生真菌与致病真菌的基因学特征及二者的关系.方法 采用溴苯提取法提取基因组DNA.以随机扩增DNA多态性法对16种支孢26株菌的DNA指纹图谱作了分析.结果 ①共选用10个随机引物进行扩增,筛选出2个具有稳定、清晰DNA扩增带型的引物,即引物2:5'-AGCGTCCTCC-3',引物5:5'-CAGCACCCAC-3'.②26株菌DNA带型不完全相同,有一定的种间和种内的遗传变异性,遗传相似性约为37.4%.③同种菌具有较高的遗传相似性.④部分腐生菌与致病菌位于同一树组中.结论 随机扩增DNA多态性法研究发现支孢属内各种间有一定的遗传相似性,但腐生菌与致病菌的DNA带型未见明显特征性差异.

关键词: 分支孢子菌属, 随机扩增多态DNA技术

Abstract: Objective To investigate the DNA polymorphism of saprophytes and pathogenicity of Cladosporium species and to find out the relationship between them.Methods The total DNA was extracted with benzyl bromide.Random amplification of polymorphic DNA(RAPD) assay was used to study the finger printing of 26 strains from 16 species.Results Of ten random primers used,two (primer 2,5) gave good reaction.The sequences of them were:primer 2:5'-AGCGTCCTCC-3',primer 5:5'-CAGCACCCAC-3'.There were some degrees of variation in RAPD patterns of 26 isolates,with interspecies and intraspecies variation.Genetic similarity of 26 strains was about 37.4%.The isolates of the same species showed high genetic similarities.Some saprophytes located in the same group of dendrogram of Cladosporium species with humanpathogenicity.Conclusion There are some genetic similarities between different species and strains,but no distinct characteristics are found between saprophytes and humanpathogens.

Key words: Cladosporium, Random amplified polymorphic DNA te chnique