中华皮肤科杂志 ›› 2001, Vol. 34 ›› Issue (5): 343-345.

• 论著 • 上一篇    下一篇

小鼠白念珠菌系统性感染中白介素4表达的研究

李家文1, 刘道凡1, 程纪群2, 邬焱卿1   

  1. 1. 华中科技大学同济医学院附属协和医院皮肤科, 武汉 430022;
    2. 北京武警总医院皮肤科
  • 收稿日期:2000-12-29 出版日期:2001-10-15 发布日期:2001-10-15

Interleukin-4in Experimental Murine Systemic Candidiasis

LI Jiawen1, LIU Daofan1, CHENG Jiqun2   

  1. Department of Dermatology, Union Hospital, Tongji Medical College of Huazhong Science and Technology University, Wuhan 430022, China
  • Received:2000-12-29 Online:2001-10-15 Published:2001-10-15

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摘要： 目的 了解白介素4(IL-4)在白念珠菌系统性感染中所起的作用.方法 建立健康小鼠及地塞米松诱导的免疫抑制小鼠系统性白念珠菌感染模型,用酶联免疫吸附试验(ELISA)及逆转录-聚合酶链反应分别检测脾内IL-4及mRNA水平,用平皿稀释法检测肾内菌落形成单位(CFU),并记录平均生存时间.结果 健康鼠致死量感染后3d、7d脾中IL-4蛋白水平分别为(124.8±24.1)pg/g、(262.8±21.8)pg/g(P<0.001),肾内带菌量分别为(21.25±6.31)×102CFU、(57.52±10.41)×102CFU(P<0.01);免疫抑制鼠组感染后3d、7d脾中IL-4蛋白水平分别为(85.3±18.8)pg/g、(140.7±37.2)pg/g(P<0.05),肾内带菌量分别为(12.00±2.80)×102CFU、(68.75±13.81)×102CFU(P<0.001).上述两组感染后3d、7d脾中mRNA水平表达趋势与其IL-4蛋白水平相似.致死量组平均生存时间16d,免疫抑制鼠组平均生存时间12.4d.结论 IL-4可能参与实验性小鼠白念珠菌系统性感染的发病.

关键词: 白细胞介素4, 念珠菌病,鼠, 念珠菌,白色

Abstract: Objective To investigate the change of interle ukin -4(IL-4) in experimental murine systemic candidiasis.Methods In the dexamethasone-induced immuno-suppressed murine systemic can didiasis models and control healthy mice,two-site ELISA and RT-PCR were applied to determine the levels of IL-4 protein or mRNA expression in the spleens respectively.Colony forming units(CFUs) of infected kidneys were determined with the plating dilution method,and the mean survival time (MST) of mice was also recorded.Results The IL-4concentration in the spleen of healthy mice was 80.1±19.1pg/g. In contrast,in those infected with lethal dose of yeasts t he concentrations of IL-4 were 124.8±24.1pg/g and 262.8±21.8pg/g on 3 days or 7 days after infection respectively.Meanwhile,the fungal lo ads in kidneys were(21.25±6.31)×102CFUs and(57.52±10.41)×102 CFUs on 3 days or 7 days after infection(P<0.001).The IL-4concentrations in the spleens of immuno-suppressed mice infected with s ublethal dose of yeasts were 85.3±18.8pg/g and 140.7±37.2pg/g on 3 day s and 7 days after infection respectively(P<0.05).The tendency of the mRNA expression on 3 days and 7 days was in accordance with the protein level.The MST of the healthy mice infected with lethal dose of yeasts was 16 days and that of the immuno-suppressed mice was 12.4 days.Conclusion IL-4may play a role in the pathogenesis of experimental murine systemic candidiasis.

Key words: Candida albicans, Interleukin-4, Candidiasis,murine