中华皮肤科杂志 ›› 2001, Vol. 34 ›› Issue (5): 333-335.

• 论著 • 上一篇    下一篇

新生隐球菌白化株酚氧化酶结构基因的突变研究

桑红1, 廖万清2, 陈江汉2, 温海2, 顾菊林2, 李林3   

  1. 1. 南京军区南京总医院皮肤科, 南京 210002;
    2. 第二军医大学附属长征医院皮肤科;
    3. 中国科学院上海生化所
  • 收稿日期:2000-12-21 出版日期:2001-10-15 发布日期:2001-10-15
  • 基金资助:
    国家自然科学基金资助课题(39870658)

CNLAC1Gene Mutation of Cryptococcus neoformans

SANG Hong1, LIAO Wanqing2, CHEN Jiang2   

  1. Department of Dermatology, Jinling Hospital, Nanjing 210002, China
  • Received:2000-12-21 Online:2001-10-15 Published:2001-10-15

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摘要： 目的 探讨新生隐球菌白化突变的分子机制,进一步研究白化突变株与野生产黑株之间漆酶基因(CNLAC1)的差异.方法 用聚合酶链反应(PCR)扩增白化突变株与野生产黑株在比较研究中发现的差异基因片段;克隆异常片段的PCR产物,用PGEM-T载体构建重组表达质粒载体,转化大肠杆菌,进行目的基因测序.结果 克隆了产黑株及白化株存在差异的片段,构建了差异基因片段的重组质粒载体,转化后测序发现在1715bp～2617bp之间白化突变株不仅存在基因片段大小的改变,而且存在碱基序列的改变.结论 新生隐球菌白化突变的分子机制,可能在于其CNLAC1基因在1715bp～2617bp之间碱基序列的改变,而不是基因片段的单纯缺失.白化突变株差异基因片段可能为新生隐球菌病的预后和治疗提供一个靶基因.

关键词: 隐球菌,新型, 漆酶基因

Abstract: Objective To explore the mechanism of Cryptococcus neoformans mutant in CNLAC1 and investigate the difference of CNLAC1 between wild type(Mel+) and the mutant (Mel-).Methods The abnormal PCR DNA product of Cryptococcus neoformans was cloned into sequencing vector PG EM-T for sequencing.An expression vector was constructed for efficient expression in E.coli DH5α.The gene was sequenced.Results The cloned DNA sequence was found to be different between wild type(Mel+) and the mutant (Mel-)(between 1715 bp and 2617 bp).The differences were not only in length,but also in base sequence.Conclusion The mechanism of the mutant in CNLAC1 might be a change of sequence(between 1715 bp and 2617 bp),and not a DNA deletion.This finding might provide us a target gene for the prognosis and treatment of cryptococcosis.

Key words: Cryptococcus neoformans, CNLAC1