中华皮肤科杂志 ›› 2001, Vol. 34 ›› Issue (3): 206-208.

• 临床经验 • 上一篇    下一篇

基底细胞癌Bcl-2、Bax、Bcl-xl蛋白免疫组化表达的定量研究

邓列华1, 赵刚1, 夏潮湧2, 黄中新2, 陆涛1, 林泽1   

  1. 1. 暨南大学医学院第一附属医院皮肤科, 广州510630;
    2. 暨南大学医学院组胚教研室
  • 收稿日期:2000-11-13 出版日期:2001-06-15 发布日期:2001-06-15
  • 基金资助:
    暨南大学医学院科研课题项目(98027)

Quantitative Analysis of Bcl-2, Bax, Bcl-xl Expression in Basal Cell Carcinoma with Immunohistochemistry

DENG Liehua1, ZHAO Gang1, XIA Chaoyong2   

  1. Department of Dermatology, First Affliated Hospital, Medical College of Jinan University, Guangzhou 510630, China
  • Received:2000-11-13 Online:2001-06-15 Published:2001-06-15

摘要: 目的 通过对基底细胞癌Bcl-2、Bax、Bcl-xl蛋白表达的研究,探讨3种蛋白在基底细胞癌发生、发展中的作用。方法 按病理亚型将46例基底细胞癌分为非侵袭组(21例)和侵袭组(25例),采用免疫组化SP法检测Bcl-2、Bax、Bcl-xl蛋白在石蜡切片上的表达,并对组化结果进行图像分析以获得平均光密度和Bcl-2/Bax的比值。结果 非侵袭性基底细胞癌组Bcl-2蛋白表达水平和Bcl-2/Bax的比值高于侵袭性基底细胞癌组,P值分别为0.005和0.044;两组间Bcl-xl、Bax蛋白表达水平的差异无显著性,P值分别为0.097和0.979。结论 Bcl-2、Bcl-xl、Bax蛋白可能参与了基底细胞癌的发生、发展。

关键词: 基底细胞癌, 基因,Bcl-2, 基因,Bax, 基因,Bcl-xl

Abstract: Objective To explore the roles of Bcl-2, Bax, Bcl-xl proteins in the carcinogenesis of basal cell carcinoma(BCC). Methods Forty-six BCC cases were divided into 2 groups (21 in non-aggressive BCC group and 26 in aggressive BCC group) according to their pathological features. Bcl-2, Bax and Bcl-xl were evaluated in formalin fixed, paraffin embedded specimens by immunohistochemical methods and analyzed by image-analysis methods to obtain average optical density (AOD) of each protein for each case. The AOD data of 3 proteins and Bcl-2/Bax ratios were then compared between the 2 BCC groups. Results The level of Bcl-2 expression and Bcl-2/Bax ratio of non-aggressive BCC group were higher than those of aggressive group,(P=0.005 and 0.044, respectively). For the expression level of Bcl-xl and Bax, there was no statistically difference between the two groups (P=0.097 and 0.979, respectively). Conclusion Bcl-2, Bax,Bcl-xl proteins might participate in the carcinogenesis and development of BCC.

Key words: Basal cell carcinoma, Genes,bcl-2, Genes,bcl-x1, Genes,bax