中华皮肤科杂志 ›› 2001, Vol. 34 ›› Issue (3): 198-201.

• 论著 • 上一篇    下一篇

豚鼠生殖器疱疹模型及细胞培养法观察中药抗病毒胶囊的抗病毒作用

范瑞强1, 李红毅1, 谢长才1, 禤国维1, 朱宇同2   

  1. 1. 广东省中医院皮肤科, 广州510120;
    2. 广州中医药大学
  • 收稿日期:2000-07-13 出版日期:2001-06-15 发布日期:2001-06-15
  • 基金资助:
    国家中医药管理局科研基金资助课题(97Z077)

Antiviral Effect of Antivirus Capsule Containing Chinese Medicinal Herbs on Guinea Pig Model of Genital Herpes and Cell Culture

FAN Ruiqiang1, LI Hongyi1, XIE Changcai1   

  1. Department of Dermatology, The Guangdong Provincal Hospital of T C M, Guangzhou 510120, China
  • Received:2000-07-13 Online:2001-06-15 Published:2001-06-15

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摘要： 目的 探讨抗病毒胶囊治疗生殖器疱疹的作用机理。方法 用雌性豚鼠生殖道单纯疱疹病毒(HSV)2型感染的模型,采取抗病毒胶囊内服,观察豚鼠阴道内病毒滴度、外阴症状;用体外细胞培养法观察抗病毒胶囊对HSV-2的直接灭活和三种给药途径下对HSV-2的抑制作用。结果 抗病毒胶囊组豚鼠阴道内病毒滴度与阿昔洛韦组比较差异无显著性(P>0.05);外阴症状评分在第2、3、5、7、8天显著低于生理盐水对照组(P<0.05),与阿昔洛韦组大致相同(P>0.05);细胞培养结果:抗病毒胶囊1号、2号对HSV-2的最低有效抑制浓度分别0.390625mg/mL和1.5625mg/mL。结论 中药抗病毒胶囊在动物体内和体外对HSV-2均有抑制作用。

关键词: 疱疹,生殖器, 中草药, 疾病模式,动物, 细胞培养

Abstract: Objective To study the therapeutic mechanism of the antivirus capsule containing Chinese herbs against genital herpes. Methods The female guinea pig GH models were treated with antivirus capsule. The vaginal titer of the virus and the symptoms of vulva were observed. Cell culture was used to detect the cytopathic suppression. Results It was shown that there was no difference between antivirus capsule group and acychovir (ACV) group on the titer of virus in the vagina of guinea pig (P>0.05). The symptom score after the treatment with antivirus capsule was significantly lower than that of saline control group (P<0.05), and similar to ACV group (P>0.05). Cell culture showed that the lowest effective concentration for suppressing HSV-2 by antiviral capsule 1 and antiviral capsule 2 was 0.390625 mg/mL or 1.5625mg/mL, respectively. Conclusion Antiviral capsule containing Chinese medicinal herbs inhibits HSV-2 in vivo and in vitro.

Key words: Herpes genitalis, Chinese medicine herbs, Disease model,animal, Cell culture