中华皮肤科杂志 ›› 2001, Vol. 34 ›› Issue (3): 195-197.

• 临床经验 • 上一篇    下一篇

复发性生殖器疱疹患者外周血CD3+T细胞内细胞因子的检测

钱起丰, 郭红卫   

  1. 深圳市皮肤病研究所、性病防治研究中心, 518020
  • 收稿日期:2000-07-14 出版日期:2001-06-15 发布日期:2001-06-15

Intracellular Cytokine Detection in Peripheral Blood CD3+T Cells of Patients with Recurrent Genital Herpes

QIAN Qifeng, GUO Hongwei   

  1. Shenzhen Institute of Dermatology, Center for STD Control and Research, Shenzhen 518020, China
  • Received:2000-07-14 Online:2001-06-15 Published:2001-06-15

摘要: 目的 研究Th1/Th2型细胞因子产生在复发性生殖器疱疹(RGH)发病机理中的作用及其相互关系。方法 采用双色荧光抗体染色技术经流式细胞仪检测了20例RGH患者外周血CD3+T细胞内5种细胞因子染色阳性细胞百分率。结果 RGH患者CD3+总T细胞百分率比正常人对照明显降低(P<0.05);IL-2、IFN-γ阳性细胞百分率均显著降低(分别为P<0.02及P<0.001);IL-10、IL-12阳性细胞则明显升高(均为P<0.01);TNF-α阳性细胞则无明显改变(P>0.05)。结论 RGH患者存在着因Th1与Th2细胞间的交互异常调节所致的有关细胞因子产生失衡,表现为Th2型细胞因子(IL-10)产生过多,而Th1型细胞因子(IL-2、IFN-γ)产生过少,由此所引起的一系列细胞免疫反应抑制效应在RGH发病机理中起着重要作用。

关键词: 疱疹,生殖器, 流式细胞术, 细胞因子

Abstract: Objective To study the role of Th1/Th2 cytokine profile in the pathogenesis of recurrent genital herpes (RGH), and to find out the relationship between Th1/Th2 cytokines. Methods A two-colour immunofluorescent staining of cell surface antigen and intracellular cytokines (IL-2、IL-10、IL-12、IFN-γ and TNF-α) in CD3+T-lymphocytes of 20 patients with RGH with flow cytometric analysis were performed. Results Compared to controls, patients with RGH showed a decreased number of CD3+T cells(P<0.05), and a decreased number of IL-2-producing and IFN-γ-producing T cells(P<0.02 and P<0.001, respectively), whereas an increased number of IL-10-producing and IL-12-producing T cells(P<0.01). There was no significant difference in the number of TNF-α-producing cells between the patients and controls(P>0.05). Conclusions RGH patients seem to have a predominance of Th2 cytokine profile, which may play an important role in the pathogenesis of RGH.

Key words: Herpes genitalis, Cytokines, Flow cytometry