中华皮肤科杂志 ›› 2001, Vol. 34 ›› Issue (3): 181-183.

• 论著 • 上一篇    下一篇

细胞培养、连接酶链反应和六种聚合酶链反应试剂盒检测沙眼衣原体的比较研究

王宝玺1, 朱学骏2, 倪安平1, 叶顺章3, 乐嘉豫4, 郑和义1, 刘全中5, 王千秋3, 汤全贵4, 秦俭2   

  1. 1. 中国医学科学院、中国协和医科大学北京协和医院, 北京市100730;
    2. 北京大学第一医院;
    3. 中国医学科学院中国协和医科大学皮肤病研究所;
    4. 上海市皮肤病性病医院;
    5. 天津医科大学总医院
  • 收稿日期:2000-08-08 出版日期:2001-06-15 发布日期:2001-06-15

Comparative Study of Six Kits of Polymerase Chain Reaction Assay with Cell Culture and Ligase Chain Reaction Assay in Detection of C. Trachomatis infection

WANG Baoxi1, ZHU Xuejun2, NI Anping1   

  1. Peking Union Medical College Hospital, CAMS and PUMC, Beijing 100730, China
  • Received:2000-08-08 Online:2001-06-15 Published:2001-06-15

PDF (PC)

73

赞

摘要： 目的 与细胞培养和连接酶链反应(LCR)比较考察6种国产聚合酶链反应(PCR)试剂盒在检测性传播疾病门诊患者标本沙眼衣原体的诊断价值。方法 在北京、上海、南京、天津5家临床医院性病门诊收集到673份尿道/宫颈拭子标本,分别进行沙眼衣原体培养和PCR检测,对结果不相符合的标本采用LCR复检,将各种PCR检测结果分别与培养、LCR以及综合结果进行比较分析。结果 合格病例616例,培养法检测阳性率6.3%,PCR检测阳性率分别为23.5%～28.7%。与培养结果比较,各种PCR检测的敏感性均在90%以上,其中PCR1、PCR2和PCR5均达到100%。LCR复核标本200份,与之相比,PCR检测的敏感性为83.9%～98.6%,特异性66.7%～94.7%,YI指数0.523～0.881。其中PCR2结果符合性最好,其它依次为PCR4、PCR1、PCR5、PCR3及PCR6。综合分析证明国产PCR检测沙眼衣原体的敏感性均在85%以上,特异性均在95%以上。YI指数由高到低分别为PCR2、PCR1、PCR3、PCR5、PCR4、PCR6。结论 国产PCR检测尿道/宫颈拭子沙眼衣原体具有较高的敏感性与特异性,可以用于临床检验,实验室质控与监督是本方法得以正确应用的关键。

关键词: 沙眼衣原体, 聚合酶链反应, 连接酶链反应, 培养

Abstract: Objective To evaluate the diagnostic value of six home made PCR kits for the detection of Chlamydia trachomatis in patients with sexually transmitted diseases, cell culture and LCR were used as references. Methods Endocervical or urethral swab specimens were collected from 673 patients attending STD clinics in Beijing, Shanghai, Nanjing and Tianjin. C. trachomatis culture and PCR were performed with specimens from all patients while LCR was performed only with specimens which the culture and PCR gave discrepant results. Results Among 616 eligible patients thirty-nine (6.3%) cases were culture positive, and PCRs revealed positive rates from 23.5% to 28.7%. In comparison with cell culture, the sensitivity of PCRs were 90% or higher. In 200 cases which the culture and PCR gave discrepant results, LCR and PCR showed excellent consistency (YI index: 0.523～0.881), the sensitivity and specificity of PCRs ranged from 83.9%～98.6% and 66.7%～94.7% respectively, while PCR2 showed the highest YI index (0.881). With an expanded gold standard for discrepant results, we found that the specificity and sensitivity of PCRs were higher than 95% and 85%, respectively. Conclusions Domestic produced PCR kits for Chlamydia trachomatis detection are highly sensitive and specific, however, the quality control of laboratory still remains important in the clinical application.

Key words: Chlamydia trachomatis, Polymerase chain reaction, Ligase chain reaction, Culture