中华皮肤科杂志 ›› 2001, Vol. 34 ›› Issue (3): 178-180.

• 论著 • 上一篇    下一篇

拭子培养、尿沉渣培养和聚合酶链反应-杂交梳检测淋球菌

苏敬泽, 汤全贵   

  1. 上海皮肤病性病医院, 200050
  • 收稿日期:2000-11-20 出版日期:2001-06-15 发布日期:2001-06-15

Comparison of Swab Culture with Urinary Sediment Culture and Polymerase Chain Reaction-Hybridocomb Test for Neisseria Gonorrhoeae

SU Jingze, TANG Quangui   

  1. Shanghai Hospital for Skin Diseases and STDs, Shanghai 200050, China
  • Received:2000-11-20 Online:2001-06-15 Published:2001-06-15

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摘要： 目的 探讨非侵入性取材的尿沉渣培养和尿沉渣聚合酶链反应(PCR)-杂交梳法检测淋球菌在诊断与治疗中的意义。方法 ①以金标准的拭子培养法与以上2种方法相对照;②同一患者的标本用以上3种方法进行检测。结果 3种淋球菌的检测方法均有较高检出率,其符合率、特异性和敏感性(男性分别为94.80%、94.81%和95.35%;女性分别为94.35%、96.97%和84.10%)差异无显著性(P>0.05),而且同一患者的标本拭子培养与尿沉渣PCR-杂交梳法之间(符合率、特异性和敏感性分别为94.76%、96.02%和91.17%)亦无差异(P>0.05)。结论 非侵入性取材的尿沉渣及PCR-杂交梳法或可取代拭子培养,可广泛用于性病防治工作、临床诊断与治疗;PCR-杂交梳法可能更具优点,但鉴于我国国情,目前更应推广尿沉渣培养法检测出淋球菌。

关键词: 奈瑟氏球菌,淋病, 细胞培养, 聚合酶链反应-杂交梳

Abstract: Objective To evaluate the diagnostic and therapeutic values of two non-invasive test methods for Neisseria gonohoeae. Methods The swab culture, urinary sediment culture and" gold standard method"-swab culture were used to detect gonocococal strains. The results were analyzed to compare the validity, sensitivity and specificity of these three methods. Results There is no significant difference in the validity, sensitivity and specificity among the three methods (Male: 94.80%、94.81% and 95.35%; Female: 94.35%、96.97% and 84.10%, P>0.05, respectively). Conclusions Non-invasive urinary sediment culture and PCR-hybridocomb test may be suitable to replace the swab culture method in STD clinic, and the urinary sediment culture is highly recommended.

Key words: Neisseria gonorrhoeae, Cell culture, Polymerase chain reaction-Hybridocomb