中华皮肤科杂志 ›› 2001, Vol. 34 ›› Issue (2): 93-94.

• 论著 •    下一篇

银屑病患者血清中抗亲环素A自身抗体的初步研究

沈新, 冯信忠   

  1. 上海第二医科大学瑞金医院皮肤科 200025
  • 收稿日期:2000-11-30 出版日期:2001-04-15 发布日期:2001-04-15

Preliminary Study on Anticyclophilin A Auto-Antibodies in Sera of Patients with Psoriasis

SHEN Xin, FENG Xinzhong   

  1. Department of Dermatology, Rui Jin Hospital, Shanghai Second Medical University, Shanghai 200083, China
  • Received:2000-11-30 Online:2001-04-15 Published:2001-04-15

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摘要： 目的 检测银屑病患者血清中的抗亲环素A自身抗体(ACA)的水平。方法 从小牛胸腺中提取、纯化亲环素A。用免疫印迹法(IBT)和酶联免疫吸附试验(ELISA)两种方法同时检测银屑病患者血清中的抗亲环素A自身抗体的水平。结果 两种方法均显示银屑病患者血清中的ACA水平显著高于正常对照组。ELISA法显示患者抗体的阳性率达41.3%。银屑病进行期患者血清中的抗体水平高于静止期患者,但无统计学意义。结论 银屑病患者血清中的ACA水平明显高于正常人。

Abstract: Objective To determine the level of anticyclophilin A auto-antibodies (ACA) in sera of patients with psoriasis. Methods Cyclophilin A (CyPA) was extracted and purified from bovine thymus. The level of anticyclophilin A auto-antibodies (ACA) was detected by enzyme-linked immunosorbent assay (ELISA) and immunoblotting test (IBT) in sera of patients with psoriasis. Results It was shown that the level of ACA was significantly higher in the sera of patients with psoriasis than those in control group (IBT:P<0.01,ELISA:P<0.01). ACA was found to be positive in 41.3% of the patients by ELISA. In psoriatic group, the serum level of ACA was higher during the progressive stage than that in the stati onary stage, however, no significant difference was observed (IBT:P>0.05,ELISA:P>0.05). Conclusion The results suggest that there is autoimmunological mechanism in the pathogenesis of psoriasis.

Key words: Psoriasis, Peptidylprolyl isomerase, Autoantibodies, Cyclosporin A