中华皮肤科杂志 ›› 2001, Vol. 30 ›› Issue (1): 47-49.

• 论著 • 上一篇    下一篇

紫外线及其辐射的尿刊酸对朗格汉斯细胞功能影响的研究

毕志刚, 蒋艺, 李燕华   

  1. 南京医科大学第一附属医院皮肤科, 210029
  • 收稿日期:2000-02-17 出版日期:2001-02-15 发布日期:2001-02-15

Effects of UVB and UV irradiated UCA on the Function of Langerhans’ Cell

LI Yanhua, BI Zhigang, JIANG Yi   

  1. Department of Dermatology, First Affiliated Hospital of Nanjing Medical University, Nanjing 210029
  • Received:2000-02-17 Online:2001-02-15 Published:2001-02-15

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摘要：

目的 探讨紫外线及其辐射的尿刊酸免疫抑制作用的可能机理.方法 观察中波紫外线(UVB)及尿刊酸对豚鼠朗格汉斯细胞刺激自体淋巴细胞增殖能力的影响.结果 低剂量UVB(25J/m2)抑制率为10.5%,50J/m2抑制率为22.4%,100J/m2抑制率为54.5%,200J/m2几乎可以完全抑制朗格汉斯细胞协同刺激功能.70%顺式尿刊酸(0.2mg/mL)抑制率仅为13.4%,反式尿刊酸无抑制作用.结论 紫外线可直接抑制朗格汉斯细胞的功能,并呈剂量依赖性,这可能在UVB免疫抑制过程中起着重要的作用.经紫外线辐射的顺式尿刊酸抑制作用轻微.

关键词: 紫外线, 尿刊酸, 郎格尔汉斯细胞, 免疫抑制

Abstract:

Objective To study the immunosuppression mechanism induced by ultraviolet (UV)and cis urocanic acid. Method The auto lymphocyte proliferation test with Langerhans'cell (LC)in guinea-pig was performed. Results In exposure to low dose of UVB (25 J/m2) radiation,the inhibition rate of lymphocyte proliferation stimulated by LC was 10.5%,the inhibition rates of UVB radiation in doses of 50 J/m2 and 100 J/m2 were 22.4% or 50%,respectively. The lymphocyte proliferation was almost completely suppressed by 200 J/m2 UVB radiation,while the inhibition of LC function by cis-urocanic acid was weak. Conclusion UVB significantly inhibits LC auto-stimulation in dose-dependent way,which may play an important role in UVB induced immunosuppression.

Key words: Ultraviolet rays, Urocanic acid, Langerhans cells, Immunosuppression