中华皮肤科杂志 ›› 2001, Vol. 30 ›› Issue (1): 44-46.

• 论著 • 上一篇    下一篇

同种异体淋巴细胞与黑素细胞混合培养的实验研究

项蕾红, 郑志忠, 祝绿川, 廖康煌, 傅雯雯   

  1. 上海医科大学附属华山医院皮肤科, 200040
  • 收稿日期:2000-03-09 出版日期:2001-02-15 发布日期:2001-02-15

Allogeneic Mixed Melanocyte Lymphocyte Culture: An Experimental Study

XIANG Leihong, ZHENG Zhizhong, ZHU Luchuan   

  1. Department of Dermatology, Huashan Hospital, Shanghai Medical University, Shanghai 20040
  • Received:2000-03-09 Online:2001-02-15 Published:2001-02-15

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摘要： 目的 黑素细胞与同种异体淋巴细胞混合培养,从体外实验来研究排斥反应的可能性.方法 采用3H-dTR掺入同位素液态闪烁计数法测定淋巴细胞的转化增殖率,并用透射电镜观察混合培养后黑素细胞的超微结构.结果 黑素细胞对同种异体淋巴细胞的转化增殖率与ConA刺激淋巴细胞转化增殖的阳性对照比较,结果显示黑素细胞的促淋巴细胞转化增殖的特异性抗原作用较弱.进一步比较黑素细胞对不同病期白癜风患者淋巴细胞的影响,发现黑素细胞对活动期白癜风患者淋巴细胞的刺激作用相对较强,而稳定期患者和正常人对照组结果差异无显著性.同时电镜观察结果显示同种异体的淋巴细胞对黑素细胞无明显损伤,黑素细胞经混合培养后,细胞的形态完整,黑素合成功能正常,胞体和树突无损伤.细胞内细胞器丰富,胞浆内可见大量的不同阶段黑素小体.结论 正常人黑素细胞的促淋巴细胞转化增殖的特异性抗原作用较弱.同种异体淋巴细胞对黑素细胞超微结构无破坏作用.在进行同种异体移植时最好选择稳定期患者.

关键词: 黑素细胞, 淋巴细胞, 培养

Abstract: Objective To investigate the melanocyte lymphocyte reacion when the allogeneic lymphocytes are cultured with normal melanocytes. Methods 3H-thymidine was incorporated into the mixed culture and the transformation and proliferation rates of lymphocytes were detected by liquid scintillation counting and expressed as cpm. Electron microscopy was used to observe the ultrastructure of melanocytes after mixed culture. Results The Resultsof lymphocyte proliferation were expressed by the stimulation indexes. The stimulation indexes in active vitiligo group was significantly different from that in stable vitiligo group and normal controls. The stimulation indexes of the melanocyte stimulated group was significantly different from that of ConA stimulated group. In the mixed melanocyte lymphocyte reaction,the allogeneic lymphocytes had little effect on the melanocytes. The ultrastructure of the melanocytes in the mixed culture showed normal morphology and normal function of synthesis of melanin. Conclusion As a specific antigen in mixed melanocyte lymphocyte reaction,melanocyte has a weak effect on the lymphocytes. The melanocytes from stable stage vitiligo patients seem more suitble to be allografted.

Key words: Melanocytes, Lymphocytes, Culture