中华皮肤科杂志 ›› 2000, Vol. 33 ›› Issue (5): 308-310.

• 论著 • 上一篇    下一篇

甄氏外瓶霉的PCR-RFLP分型研究

李东明, 李若瑜, 王端礼, 王晓红, 马圣清   

  1. 北京大学第一医院皮肤科, 100034
  • 收稿日期:1999-10-18 出版日期:2000-10-15 发布日期:2000-10-15

PCR-ribotyping of Exophiala jeanselmei

LI Dongming, LI Ruoyu, WANG Duanli   

  1. Department of Dermatology, First Hospital, Peking University, Beijing 100034
  • Received:1999-10-18 Online:2000-10-15 Published:2000-10-15

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摘要： 目的 对不同来源的甄氏外瓶霉进行分析,为建立更为合理的分型方法提供依据。方法 以ITS3和ITS4为引物,对17株甄氏外瓶霉的核糖体DNA转录间隔区(ITSⅡ)进行扩增,4种内切酶(HinFⅠ、MspⅠ、BsuRⅠ和RsaⅠ)酶切。结果 ITSⅡ区扩增片段约为330bp,17个菌株可分为Ⅵ型,3株变种分属于不同的型,BMU00195带型独特,有可能为新变种或新种;种群关系与来源显示出一定的相关性。结论 PCR-RFLP用于甄氏外瓶霉的分型研究较为可靠,对于确定有争议菌种的分类学地位具有重要的意义。

关键词: 甄氏外瓶霉, 随机扩增多态DNA技术

Abstract: Objective To analyze Exophiala jeanselmei strains recovered from different sources and to establish a better method for typing the strains in this species. Methods The ribosomal internal transcribed spacer regions (ITSⅡ) of 17 isolates Exophiala jeanselmei were amplified with primer ITS3 and ITS4 and digested with four restriction enzymes (HinFⅠ, MspⅠ, BsuRⅠ and RsaⅠ). Results The amplicon length of the ITSⅡ was about 330bp, and 6 types of characteristic patterns for the species were found after restriction analysis. The variants of E. jeanselmei were unrelated and should therefore be maintained as separate species. The unique patterns of isolate BMU00195 were shown,which seems probably to be a new variant or even a new species. Conclusions PCR-RFLP is useful for the typing and identification of Exophiala jeanselmei, which is important in confirming the taxonomic position of a complex species.

Key words: Exophiala jeanselmei, Random amplified polymorphic DNA technique