中华皮肤科杂志 ›› 2000, Vol. 33 ›› Issue (4): 246-248.

• 论著 • 上一篇    下一篇

基底细胞癌、鳞状细胞癌和Bowen病9p21、9q22.2-q22.3杂合性丢失的研究

何春涤1, 张学2, 王雅坤1, 孙开来2, 陈洪铎1   

  1. 1. 中国医科大学第一临床学院皮肤科 沈阳 110001;
    2. 中国医科大学基础医学院遗传教研室
  • 收稿日期:1999-10-07 出版日期:2000-08-15 发布日期:2000-08-15
  • 基金资助:

    国家自然科学基金资助课题(39470651)

Loss of Heterozygosity on Chromosome 9p21, 9q22.2-22.3 in Basal Cell Carcinoma, Squamous Cell Carcinoma and Bowen’s Disease

HE Chundi1, ZHANG Xue2, WANG Yakun1   

  1. Department of Dermatology, No.1 Hospital, China Medical University, Shenyang 110001
  • Received:1999-10-07 Online:2000-08-15 Published:2000-08-15

PDF

71

赞

摘要：

目的 探讨9号染色体的改变在皮肤肿瘤发生过程中的作用。方法 应用D9S319(9p21)和D9S299(9q22.2-q22.3)2个座位微卫星DNA多态标记,对基底细胞癌、鳞状细胞癌和Bowen病进行了杂合性丢失分析。结果 在可提供信息的10例基底细胞癌、19例鳞状细胞癌和4例Bowen病中,均未观察到D9S319(9p21)杂合性丢失的存在;10例基底细胞癌中,有2例在9q22.2-q22.3(D9S299)位点出现杂合性丢失;因此,在9q可能存在具有潜力的抑癌基因。而在21例鳞状细胞癌及4例Bowen病中,均未观察到D9S299杂合性丢失的存在。结论 9q22.2-q22.3可能存在具有潜力的抑癌基因,其缺失在基底细胞癌的发生中起一定作用。

关键词: 癌,基底细胞, 癌,鳞状细胞, Bowen病, 杂合子丢失, 染色体,人,9对

Abstract:

Objective To investigate the role of allele loss on chromosome 9 in the pathogenesis of basal cell carcinoma, squamous cell carcinoma and Bowen's disease. Methods Two polymorphic microsatellite sequences at loci D9S319 (9p21) and D9S299 (9q22.2-q22.3) were examined by polymerase chain reaction for loss of heterozygosity. Results Loss of heterozygosity with D9S319 (9p21) marker was not observed in 10 informative cases of sporadic basal cell carcinoma, 19 cases of squamous cell carcinoma and 4 cases of Bowen's disease. Allelic deletion of D9S299 was not found in all 21 informative cases of squamous cell carcinoma and 4 cases of Bowen's disease. D9S299 (9q22.2-22.3) allele loss occurred in 2 of 10 informative cases of sporadic basal cell carcinoma, indicating that there might be a susceptible tumor suppressor gene on chromosome 9. Conclusion Our findings suggest that chromosome 9q22.2-22.3 might contain a putative tumor suppressor gene, and loss of which may play an important role in the pathogenesis of basal cell carcinoma.

Key words: Carcinoma basal cell, Carcinoma,squamous cell, Bowen’s diseas, Loss of heterozygosity, Chromosomes,human,pair 9