中华皮肤科杂志 ›› 2000, Vol. 33 ›› Issue (4): 235-237.

• 论著 • 上一篇    下一篇

毛喉素对8-甲氧补骨脂素诱导鼠黑素瘤细胞酪氨酸酶基因表达水平的调节

雷铁池, 朱文元, 夏明玉   

  1. 南京医科大学第一附属医院皮肤科 210029
  • 收稿日期:1999-08-18 出版日期:2000-08-15 发布日期:2000-08-15
  • 基金资助:
    国家自然科学基金资助项目(39870701)

Regulation of 8-methoxypsoralen-induced Tyrosinase mRNA Expression by Forskolin in B16F10 Murine Melanoma Cells

LEI Tiechi, ZHU Wenyuan, XIA Mingyu   

  1. Department of Dermatology, First Affiliated Hospital of Nanjing Medical University, Nanjing 210029
  • Received:1999-08-18 Online:2000-08-15 Published:2000-08-15

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摘要： 目的 观察蛋白激酶A信号途径对8-甲氧补骨脂素(8-MOP)诱导的鼠黑素瘤细胞黑素生成及酪氨酸酶基因表达水平的影响。方法 用半定量逆转录聚合酶链反应技术检测腺苷酸环化酶特异性激活剂毛喉素对8-MOP诱导的鼠黑素瘤细胞酪氨酸酶活性、黑素含量以及酪氨酸酶基因表达水平的影响。结果 毛喉素与8-MOP联合处理组所测的酪氨酸酶活性和黑素含量与空白对照组相比提高近7或10倍。毛喉素与8-MOP单独处理组较空白对照组酪氨酸酶mRNA表达水平上调百分率分别为26.06%和6.58%,而联合处理组上调百分率为56.82%。结论 毛喉素在体外对8-MOP诱导的黑素生成呈明显增强作用,这一作用与毛喉素在转录水平上调酪氨酸酶mRNA表达促进酶蛋白合成有关。毛喉素作为治疗白癜风的一种信号转导药物,其临床应用价值有待今后进一步研究。

关键词: 一元酚单氧酶, 环AMP依赖性蛋白激酶类, 基因表达, 甲氧沙林

Abstract: Objective To explore the regulatory mechanism of tyrosinase activity by cAMP/PKA dependent signaling pathway. Methods The regulatory effects on tyrosinase mRNA level were assessed by forskolin, a specific activator of adenylate cyclase in cultured B16F10 murine melanoma cells by using RT-PCR technique. Results It was shown that forskolin could significantly enhance tyrosinase activity, melanin content, and tyrosinase mRNA level. Tyrosinase activity and melanin content were as much as seven to ten-fold higher in cells exposed simultaneously to forskolin and 8-MOP than those in untreated cells. The levels of tyrosinase mRNA were up-regulated by 26.06% with 8-MOP, 6.58% with forskolin, and 56.82% with 8-MOP plus forskolin, respectively. Conclusion Forskolin is shown to enhance 8-MOP-induced tyrosinase activity, probably by way of up-regulation of tyrosinase mRNA expression and tyrosinase synthesis. Further study is needed to understand the therapeutic significance of forskolin for vitiligo.

Key words: Monophenol monooxygenase, Cyclic AMP-dependent protein kinases, Gene expression, Methoxsalen