中华皮肤科杂志 ›› 2000, Vol. 33 ›› Issue (2): 99-101.

• 论著 • 上一篇    下一篇

表皮生长因子对游离毛囊生长的影响

杨淑霞, 钟志红, 秦俭, 马圣清, 武玲慎   

  1. 北京医科大学第一医院皮肤科 100034
  • 收稿日期:1999-05-31 发布日期:2000-04-15
  • 基金资助:
    宝洁公司资助课题

Effects of Epidermal Growth Factor on Human Hair Follicle Growth in vitro

YANG Shuxia, ZHONG Zhihong, QIN Jian   

  1. Department of Dermatology, The First Hospital of Beijing Medical University, Beijing 100034
  • Received:1999-05-31 Published:2000-04-15

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摘要： 目的 通过毛囊器官培养观察表皮生长因子(EGF)对人头皮毛囊生长的影响。方法 用WilliamsE无血清培养基进行人头皮毛囊游离培养,分为2组,EGF组(43根)和对照组(43根)。结果 EGF20μg/L组在加药后1～3d毛干平均生长0.22mm/d,对照组平均生长0.16mm/d,两组差异有显著性(P<0.05),EGF对毛囊生长有更明显的促进作用。但EGF组毛囊于培养的第4～6天即出现退行期的形态改变并发生扭曲,毛干停止生长,对照组于培养第10天后才发生形态变化。组织学上,培养第10天EGF组毛囊下部外毛根鞘增厚,杵状结构形成,而对照组仍为生长期形态。3H-亮氨酸掺入实验显示EGF组毛囊上皮中的放射活性(370c/min)高于对照组(271.14c/min)(P<0.05),而毛干中的放射活性(173.19c/min)与对照组(158.49c/min)无明显差异。结论 EGF对毛囊的生长有促进作用,主要是促进毛囊上皮的生长,并且诱导毛囊提前由生长期进入退行期。

关键词: 毛囊, 器官培养, 表皮生长因子-尿抑胃素

Abstract: Objective To investigate the effects of epidermal growth factor (EGF) on hair follicle growth. Methods Cultured human hair follicles were divided into two groups:EGF-treated group (EGF 20 ng/mL) and control group. The length and morphology of follicles were measured by inverted microscope daily. 3H-leucine incorporation and histologic studies were applied. Results The rates of hair shaft and hair follicle elongation significantly increased in the EGF-treated group during day 1 to day 3 in comparison with the control group. Histologically, the cells of the lower outer root sheath were thickened and club hair-like structure was formed. 3H-leucine incorporation showed that the radioactivity in hair follicle epithelium was higher in EGF-treated group (370 c/min)than that in control group(271.14 c/min) (P<0.05). No difference was found in terms of the radioactivity in hair shaft between EGF-treated group(173.19 c/min) and control group(158.49 c/min). Conclusion It is suggested that EGF might stimulate the growth of hair follicle epithelium, and induce anagen transforming into catagen prematurely.

Key words: Hair follicle, Organ culture, Epidermal growth factor-urogastrone