中华皮肤科杂志 ›› 2000, Vol. 33 ›› Issue (2): 96-98.

• 论著 • 上一篇    下一篇

雷公藤内酯醇对体外培养的成骨细胞增殖的影响

黄岚1, 冯树芳1, 王洪复2, 金慰芳2, 魏道林2, 高建军2   

  1. 1. 上海医科大学华山医院皮肤科 200040;
    2. 上海医科大学放射医学研究所
  • 收稿日期:1999-04-25 发布日期:2000-04-15

Effects of Triptolide on the Proliferation Capacity of Osteoblasts in vitro

HUANG Lan1, FENG Shufang1, WANG Hongfu2   

  1. Department of Dermatology, Huashan Hospital, Shanghai Medical University, Shanghai 200040
  • Received:1999-04-25 Published:2000-04-15

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摘要： 目的 观察雷公藤内酯醇(TL)对体外培养成骨细胞增殖的影响,以进一步探讨雷公藤致女性骨质疏松的机制。方法 以Sprague-Dauley(SD)大鼠成骨细胞增殖为模型,分离新生SD大鼠头盖骨成骨细胞,传代后加入不同浓度TL。每浓度组设4个复孔,以PBS作阴性对照,用MTT比色分析法检测TL在体外对成骨细胞增殖的作用和效价。结果 培养2d,10ng/mLTL显著抑制成骨细胞增殖(P<0.01);培养4d,0.01ng/mLTL显著抑制成骨细胞增殖(P<0.01)。药物对成骨细胞增殖的抑制程度与其剂量呈正相关(P<0.05)。结论 TL微量时即显著抑制成骨细胞增殖,且呈剂量依赖性。雷公藤对成骨细胞的直接抑制作用是其导致药物性骨质疏松的一个重要原因。

关键词: 雷公藤内酯, 成骨细胞

Abstract: Objective The effects of Triptolide(TL) on the proliferation capacity of osteoblasts were studied in order to further explore the mechanism of osteoporosis induced by Tripterygium Wilfordii Hook.F (TW). Methods Osteoblasts were obtained from the calvaria of new-born SD rats and were cultured with various concentrations of TL. The effects of TL on proliferation capacity of osteoblasts were detected by MTT methods. Results The proliferation rates of osteoblasts cultured for 2 days and 4 days were significantly inhibited by 10 ng/mL and 0.01 ng/mL of TL respectively. The inhibition rate was positively correlated with TL concentrations. Conclusion It is indicated that the direct suppressive effects of TL on proliferation capacity of osteoblasts play an important role in the pathogenesis of osteoporosis induced by TW.

Key words: Triptolide, Osteoblast