中华皮肤科杂志 ›› 1999, Vol. 32 ›› Issue (5): 319-322.

• 论著 • 上一篇    下一篇

彗星测定法检测蕈样肉芽肿和S-zary综合征的DNA不稳定性

汪晨1, Marianne Lind2, Karen Bang2, Kristian2, Thestrup-Pedersen2   

  1. 1. 中日友好医院皮肤科 北京 100029;
    2. The Department of Dermatology, University of Aarhus, Denmark
  • 收稿日期:1998-12-01 修回日期:1999-04-07 出版日期:1999-10-15 发布日期:1999-10-15

The DNA Instability in Mycosis Fungoides and Sezary’s Syndrome by Using the Comet Assay

WANG Chen1, Marianne Lind2, Karen Bang2   

  1. Department of Dermatology, China Japan Friendship Hospital, Beijing 100029
  • Received:1998-12-01 Revised:1999-04-07 Online:1999-10-15 Published:1999-10-15

摘要:

目的 研究蕈样肉芽肿 (MF)和S啨zary综合征 (SS)患者淋巴细胞DNA的不稳定性.方法采用彗星测定 (Comet Assay)或称为单一细胞凝胶电泳 (The single cell gel electrophoresis SCGE)研究受X线引起MF和SS患者外周血淋巴细胞、皮肤损害的淋巴细胞培养建立的T细胞系的DNA损伤和修复.以正常人淋巴细胞为对照组.结果 MF和SS患者淋巴细胞DNA有明显的不稳定性,且很难修复,与对照组差异有显著性.结论MF和SS患者淋巴细胞的DNA表现明显的不稳定性和修复障碍,提示MF和SS是从一种良性皮肤病发展成恶性肿瘤.

关键词: 彗星测定法, 蕈样肉芽肿, S-zary’s综合征, DNA不稳定性

Abstract:

Objective To study the DNA instability of the peripheral blood lymphocytes and T-cell line from skin lesions of mycosis fungoides(MF) and S閦ary's syndrome (SS).Methods The Comet assay,which was also called as SCGE (the single cell gel electrophoresis),was used to detecting the primary DNA damage at the level of single cell in specimens.The DNA damage of X-ray-irradiated peripheral blood lymphocytes and T lymphocytes from skin lesions,and its repair in lymphoma patients were studied,and compared with those in control persons.Results A significantly increased DNA instability in the majority of lymphocytes from the blood and skin lesions of MF and SS patients was observed,and reduced DNA repair of X-ray irradiated lymphocytes was found,when compared with the lymphocytes from healthy individuals.Conclusion The DNA stability and repairment of both blood and skin-homing lymphocytes from patients with MF and SS are significantly impaired,which suggests that MF and SS could develop from a clinically benign skin disease into an overt lymphoma.

Key words: Comet assay, Mycosis fungoides, Sezary’s Syndrome, DNA Instability