中华皮肤科杂志 ›› 1999, Vol. 32 ›› Issue (5): 309-312.

• 论著 • 上一篇    下一篇

奶粉吐温琼脂鉴定白念珠菌的实验研究

吕桂霞, 沈永年, 秦振宇, 吴绍熙   

  1. 中国医学科学院、中国协和医科大学皮肤病研究所 南京 210042
  • 收稿日期:1999-01-05 修回日期:1999-03-24 出版日期:1999-10-15 发布日期:1999-10-15

Identification of Candida albicans by Using Milk Tween Agar

LÜ Guixia, SHEN Yongnian, QIN Zhenyu   

  1. Institute of Dermatology, Chinese Academy of Medical Sciences, Peking Union Medical College, Nanjing 210042
  • Received:1999-01-05 Revised:1999-03-24 Online:1999-10-15 Published:1999-10-15

摘要: 目的 改进白念珠菌鉴定方法.方法 设计奶粉吐温琼脂 (MTA)可一次鉴定白念珠菌的芽管和顶端厚壁孢子,并选择来源不同的103株念珠菌 (白念珠菌88株,克柔念珠菌、季也蒙念珠菌、近平滑念珠菌、热带念珠菌和乳酒念珠菌各3株)与灭活人血清和玉米吐温琼脂相比较.结果 88株白念珠菌在MTA上能产生芽管的有69株,在灭活人血清上有78株.MTA 24h能产生顶端厚壁孢子的菌株占 85.2%,明显优于玉米吐温琼脂 (46.6%),48h和72h两者间无差异.结论 MTA产生芽管和顶端厚壁孢子的能力不低于传统方法,且操作更为简便,在实际工作中有良好的应用前景.

关键词: 白念珠菌, 厚壁孢子, 芽管

Abstract: Objective To improve the method of identifying Candida albicans.Methods A new medium consisting of milk powder,Tween 80 and agar(milk tween agar,MTA) was designed for the development of both germ tube and chlamydoconidia by C-albicans In total 103 isolates,including C-albicans(88),C-guilliermondii(3),C-krusei(3),C-parapsilosis(3),C-tropicalis(3),and C-keytr(3) were cultured on this medium,compared with those on corn meal tween agar medium or inactivated human sera.Results The germ tube production by C-albicans was seen in 69 out of 88 strains on MTA medium C-albicans was found to be able to produce a higher percentage of chlamydoconidia(85.2%) after 24 hour incubation in MTA medium than in the conventional corn meal tween 80 medium(46.6%).Conclusion The new medium gives a signficantly higher percentage of germ tube and chlamydoconidia production,and can be used in the clinical mycology laboratory.

Key words: Candida albicans, Chlamydoconidia Germ, tube