细胞毒T细胞识别皮肤T细胞淋巴瘤的T细胞受体个体基因型肽抗原

中华皮肤科杂志 ›› 1999, Vol. 32 ›› Issue (1): 36-39.

细胞毒T细胞识别皮肤T细胞淋巴瘤的T细胞受体个体基因型肽抗原

丁铁钢¹, 邱丙森², Lynda Tyrell¹, Jack Longley¹

1. 美国耶鲁大学医学院皮肤科;
2. 上海医科大学皮肤病学研究所

收稿日期:1997-08-01 修回日期:1998-05-08 出版日期:1999-02-15 发布日期:1999-02-15

Recognition of Cutaneous T Cell Lymphoma T Cell Receptor Idiotypic Peptide Antigens by Cytotoxic T Cells

DING Tiegang¹, QIU Bingsen², Lynda Tyrell¹

1. Department of Dermatology, Medical College, Yale University USA;
2. Department of Dermatology, Huashan Hospital, Shanghai Medical University, Shanghai 200040

Received:1997-08-01 Revised:1998-05-08 Online:1999-02-15 Published:1999-02-15

摘要/Abstract

摘要： 目的确定细胞毒T细胞是否能识别由第三互补决定区特异基因编码的肽.方法选择两例皮肤T细胞淋巴瘤(CTCL)患者(SS和AR)的CD4⁺、Vβ8⁺恶性T细胞作为测定肽序列的细胞,CD8⁺非恶性T细胞作为杀伤或反应性细胞,和转化的淋巴母细胞样细胞作为主要组织相容性复合体(MHC)抗原提呈的细胞.结果患者SS的恶性T细胞的T细胞受体(TCR)β链的个体基因型肽可刺激CD8⁺T细胞产生肿瘤坏死因子(TNF)α,但患者AR的MTC的TCR蛋白的个体基因型区的对照肽则不刺激产生TNFα.反之,患者AR的CD8⁺T细胞藉分泌TNFα而对自身肽应答,但对来源于对照患者SS和人类免疫缺陷病毒感染患者的其他肽不应答.结论细胞毒T细胞对CTCL的淋巴细胞个体恶性克隆独特的特殊的MHCⅠ类相关肽的识别和应答具有特异性,确定CTCL的肿瘤特异性定位,可进一步为能够激发对肿瘤细胞的CD8⁺T细胞参与的免疫应答的疫苗研制和治疗提供新线索.

关键词: 淋巴瘤,T细胞,皮肤, 受体,抗原,T细胞

Abstract: Objective To determine whether cytotoxic T cells could recognize the peptides encoded by specific gene of the third complementary determining regions(CDR3).Methods The cells separated from two patients with cutaneous T cell lymphoma(CTCL) (SS and AR) CD4⁺ Vβ 8⁺ malignant T cells(MTC) were used as peptide sequence determining cells,CD8⁺ non malignant T cells as killer cells or reactive cells,and transformed lymphoblastoid cells as major histocompatibility complex(MHC) antigen presenting cells.Results The idiotypic peptide from patient SS's TCRβ chain of MTC could stimulate tumor necrotizing factor (TNF)-α production by autologous CD8⁺ T cells.TNF-α production was not stimulated by a control peptide derived from the idiotypic region of patient AR's TCR protein of MTC.In contrast,the CD8⁺ T cells of patient AR responded to autologous peptide by secreting TNF-α,but did not respond to other peptides derived from the control patient SS and patients with HIV infection.Conclusion Cytotoxic T cells may recognize and respond to a specific MHC class I associated peptide unique to individual malignant clones of lymphocytes in CTCL.The identification of a tumor specific epitope in this disease may permit the development of vaccines that could boost or initiate the CD8⁺ T cells mediated immune response to the tumor cells.

Key words: Lymphoma,T-cell,cutaneous, Receptors,antigen

丁铁钢, 邱丙森, Lynda Tyrell, Jack Longley. 细胞毒T细胞识别皮肤T细胞淋巴瘤的T细胞受体个体基因型肽抗原[J]. 中华皮肤科杂志, 1999, 32(1): 36-39.doi:

DING Tiegang, QIU Bingsen, Lynda Tyrell. Recognition of Cutaneous T Cell Lymphoma T Cell Receptor Idiotypic Peptide Antigens by Cytotoxic T Cells[J]. Chinese Journal of Dermatology, 1999, 32(1): 36-39.doi:

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