中华皮肤科杂志 ›› 1999, Vol. 32 ›› Issue (1): 28-30.

• 论著 • 上一篇    下一篇

小鼠触须毛囊体外培养的研究

范卫新, 朱文元, 雷铁池   

  1. 南京医科大学第一附属医院皮肤科 210029
  • 收稿日期:1998-04-16 修回日期:1998-09-11 出版日期:1999-02-15 发布日期:1999-02-15
  • 基金资助:
    中华医学会皮肤病学分会的资助

Study on Organ Culture of Mouse Vibrissa Follicle in Vitro

FAN Weixin, ZHU Wengyuan, LEI Tiechi   

  1. Department of Dermatology, First Affiliated Hospital, Nanjing Medical University, Nanjing 210029
  • Received:1998-04-16 Revised:1998-09-11 Online:1999-02-15 Published:1999-02-15

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摘要： 目的 为了建立较理想的小鼠触须毛囊体外培养模型,对小鼠触须毛囊体外培养方法进行了研究.方法 通过小鼠触须毛囊体外无血清培养基的方法,对不同鼠龄及不同生长周期的毛囊进行研究.结果 ①毛囊在WilliamsE加L-谷酰胺培养基上生长平均达8天;②通过对比不同阶段的生长期毛囊发现生长期Ⅱ毛囊生长力明显优于其它各期毛囊;③对不同鼠龄毛囊体外培养生长状况的研究发现出生35天和65天鼠毛囊优于出生7天乳鼠毛囊,而出生35天与65天鼠间无显著性差异;④研究还发现将触须毛囊上1/3切除之后毛囊生长优于毛囊上1/3切断后混合培养组及完整毛囊培养组.结论 Williams'E加L-谷酰胺是小鼠触须毛囊体外培养较适宜的培养基.成年小鼠生长期Ⅱ触须毛囊是较好的毛囊体外培养模型.培养中切去毛囊上1/3可促进毛囊生长.

关键词: 生长因子, 毛囊, 培养

Abstract: Objective To study and establish a culture model of mouse vibrissa follicle in vitro.Methods Mouse vibrissa follicles cultured in serum free medium were used to study the follicles in different ages of mice and in different growing stages Results 1 With Williams medium E and L-glutamine,the follicles kept growing for more than 8 days 2 Follicles in the early phase of anagen (anagen-II)grew significantly faster than the follicles in any other phases,3 The growth of hair fibers was better in adult mice than that in 7-day-old mice.4 The follicles after excising the upper one third grew faster than a mixture of two parts of cut follicles and the intact follicles.Conclusion 1 Mouse vibrissa follicles in early anagen grew better than that in any other phases of the hair cycle 2 With Williams medium E plus L-glutamine,the mouse follicles kept growing over 8 days.3 Cutting off the upper one third of the follicle during the culture promotes the growth of the follicle.

Key words: Growth factor, Follicles, Culture