中华皮肤科杂志 ›› 1999, Vol. 32 ›› Issue (1): 22-24.

• 论著 • 上一篇    下一篇

白介素6基因给药对BXSB狼疮小鼠发病的影响

杨高云1, 刘红涛2, 李世荫1, 姜学义1, 宋泉声2, 马大龙2   

  1. 1. 北京医科大学第三医院皮肤科 100083;
    2. 北京医科大学分子免疫实验室
  • 收稿日期:1997-12-22 修回日期:1998-04-17 出版日期:1999-02-15 发布日期:1999-02-15

Acceleration of Murine Lupus Disease Activity in BXSB Mice by IL-6 Gene Delivery

YANG Gaoyun1, LIU Hongtao2, LI Shiyin1   

  1. Department of Dermatology, Third Teaching Hospital, Beijing Medical University, Beijing 100083
  • Received:1997-12-22 Revised:1998-04-17 Online:1999-02-15 Published:1999-02-15

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摘要： 目的 为了进一步观察白介素6(IL6)在系统性红斑狼疮(SLE)发病中的作用.方法 采用基因工程方法构建了真核表达载体pcDNA3IL6,并经体内和体外实验检测到被其转染的细胞高效表达IL6和IL6mRNA.先后2次(于第1、第20天)将这种质粒多点肌注于12只35月龄雄性BXSB狼疮小鼠,每次每只100μg,同时用等量空载体质粒(pcDNA3)肌注于10只同龄雄性BXSB狼疮小鼠作对照.结果 发现IL6在该鼠体内的过表达导致其血清ANA滴度、尿蛋白水平和肾小球IgG、C3沉积较对照组明显增加,血清IL6水平和肾小球IL6表达高于对照组,并有2例小鼠脾脏明显增大.结论 证实了IL6在SLE发病中的病理性作用,并提示阻止或抑制IL6的产生或分泌可能有助于SLE的治疗.

关键词: 红斑狼疮,系统性, 白细胞介素6, BXSB小鼠

Abstract: Objective To study the role of IL-6 in the pathogenesis of SLE.Methods Eukaryotic expression vector pcDNA3-IL-6 encoding for IL-6 was constructed,and intramuscularly injected into 12 of 3.5-month-old-male BXSB mice at a dose of 100μg per mouse at an interval of 20 days,and 10 male BXSB mice with same age were injected plasmid pcDNA3 at the same time as control Serum ANA titer and proteinuria were monitored every 10 days,serum level of IL-6,IgG,C3 deposition and IL-6 expression on kidney were detected at the 40th day.Results IL-6 encoding plasmid had harmful effect on murine SLE with increased ANA level,proteinuria,IgG,C3 deposition and IL-6 expression on kidney compared with those of the control mice,but there was no difference on serum GPT levels between the two groups.Conclusion These results support the concept that excessive production of IL-6 might play an important role in the pathogenesis of SLE,which suggests that blocking or inhibiting the production or secretion of IL-6 might be of therapeutic value in SLE.

Key words: Lupus erythematosus,systemic, Interleukin-6, BXSB mice