中华皮肤科杂志 ›› 1998, Vol. 31 ›› Issue (6): 371-372.

• 论著 • 上一篇    下一篇

米诺地尔对培养鼠触须毛囊生长的影响

傅琳玲, 朱文元, 范卫新   

  1. 南京医科大学第一附属医院皮肤科 210029
  • 收稿日期:1997-12-29 修回日期:1998-02-28 出版日期:1998-12-15 发布日期:1998-12-15

Study on the Effect of Minoxidil on Mouse Vibrissae Follicles in Vitro

Fu Linling, Zhu Wenyuan, Fan Weixin   

  1. Department of Dermatology, The First Affiliated Hospital, Nanjing Medical University, Nanjing 210029
  • Received:1997-12-29 Revised:1998-02-28 Online:1998-12-15 Published:1998-12-15

PDF

281

赞

摘要： 目的 体内米诺地尔临床应用引起多毛, 局部外用治疗秃发有效 方法 采用C57BL/6J近交系乳鼠触须毛囊培养、毛囊显微形态观察及3H-TdR同位素标记掺入等技术, 研究在体外不同浓度米诺地尔在不同培养时间条件下对毛囊生长的影响.结果 米诺地尔浓度70μg/ml, 培养24小时, 毛囊生长较快.毛囊形态学改变示毛乳头松散, 毛球部扩大, 毛母质细胞增生活跃, 培养48小时, 毛囊生长明显减慢.培养30小时, 3H-TdR摄入率明显增高.结论 米诺地尔对体外培养鼠触须毛囊有直接促生长作用, 且与米诺地尔浓度及培养时间有一定关系.

关键词: 米诺地尔, 毛发, 组织培养

Abstract: Objective To study the effect of minoxidil on the growth of hair follicles.Methods Organ cuhure of new-born mouse vibrissa follicles in vitro.incorportating of 3H-TdR radioaetive thymidine into the cultured follieles and the histopathologic features of the follicles.Results When the the follieles were eultured in 701μg/ml of minoxidil for 24h.the follicles had a signifieanl inerease in length, the hair dermal papilla loosned. the follicle bulb enlarged, and the matrix cells grew actively, when cultured for 48h.Ihe rate of increase of the length of the follicles reduced.When cultured for 30h.there was a signfieant increase of 3H-TdR incorporating. Conclusion The results indieate thai minoxidil promotes the growth of hair follicles, and is related to its CODcentration and the duration of culture of the follieles.

Key words: Minoxidil, Hair, Tissue culture