中华皮肤科杂志 ›› 1998, Vol. 31 ›› Issue (5): 302-303.

• 论著 • 上一篇    下一篇

男性无症状性病高危人群尿沉渣检测沙眼衣原体

谢礼豪, 程文海, 黄澍杰, 周康涛, 卢和琨, 吴志周, 戴丽华   

  1. 广东省江门市皮肤病性病防治研究所, 529000
  • 收稿日期:1997-09-22 修回日期:1998-02-12 出版日期:1998-10-15 发布日期:1998-10-15

Detection of Chlamydia Trachomatis in Urine from Asymptomatic Males of STD High Risk Population

Xie Lihao, Cheng Wenhai, Huang Shujie   

  1. Jiangmen Institiute of Dermatology, Center for STD Prevention and Treatment, Jiangmen, Guangdong 529000
  • Received:1997-09-22 Revised:1998-02-12 Online:1998-10-15 Published:1998-10-15

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摘要： 目的 研究能否用尿沉渣代替尿道拭子检测沙眼衣原体(Ct).方法 分别用Wellcozyme chlamydia法和PCR法对352例男性无症状的性病高危人群首段尿沉渣进行Ct检测,两法经尿沉渣、尿道拭子配对研究。结果 以尿道拭子Ct培养为金标准,两种方法的敏感性和特异性分别为50.98%(P<0.05)和100%(P>0.05),98.04%(P>0.05)和96.68%(P>0.05).结论 对Ct的检测,用Wellcozyme chlamydia法时,不宜用尿沉渣代替尿道拭子作标本;而用PCR法时,可用尿沉渣代替尿道拭子作标本。

关键词: 沙眼衣原体, 聚合酶链反应, 酶联免疫反应

Abstract: Objective To study whether Chlamydia trachomatis (Ct) can be detected in urine specimens. Methods The first void urine sediments from 352 asymptomatic males of STD high risk population were measured by Wellcozyme chlamydia and PCR. Results Using the urethral swab culture as gold standard, the sensitivity and specificity of Wellcozyme chlamydia were 50.98% (P<0.05) and 100% (P>0.05), and those of PCR were 98.04% (P>0.05) and 96.68% (P>0.05), respectively. Conclusion It is suggested that urine sediments can not be used as the specimens for the detection of Ct by Wellcozyme chlamydia, and urine sediments can be used if Ct is detected by PCR.

Key words: Chlamydia trachomatis, Polymerase chain reaction, Enzyme immunoassay