中华皮肤科杂志 ›› 1998, Vol. 31 ›› Issue (3): 168-170.

• 论著 • 上一篇    下一篇

一例复发性生殖器疱疹的临床及其致病的HSV2野生株的糖蛋白D基因序列分析

杨慧兰1, 廖元兴1, 李明2, 江悦华2, 吴锦银2, 徐广坤3   

  1. 1. 广州军区总医院皮肤科 510010;
    2. 广州军区总医院医学实验科 510010;
    3. 中山医科大学附属第三医院皮肤科
  • 收稿日期:1997-06-05 修回日期:1997-09-15 出版日期:1998-06-15 发布日期:1998-06-15
  • 基金资助:
    广东省自然科学基金资助(960671)

Clinical Feature and Glycoprotein D Gene Sequence Analysis of a Wild Strain of HSV-2 Isolated from One Relapsed Patient with Genital Herpes

Yang Huilan1, Liao Yuangxing1, Li Ming2   

  1. Guangzhou General Hospital of Guangzhou Military Command, Guangzhou 510010
  • Received:1997-06-05 Revised:1997-09-15 Online:1998-06-15 Published:1998-06-15

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摘要： 目的 探讨我国复发性生殖器疱疹的临床表现与糖蛋白D基因序列之间的关系.方法 利用聚合酶链反应从我国一例复发性生殖器疱疹患者分离的野生株中扩增、克隆出糖蛋白D(gD)基因序列.结果 将野生株gD基因序列同国外已发表的疱疹病毒.2G株进行比较,发现其gD基因的氨基酸序列有个别突变.结论 本课题对研究我国复发性生殖器疱疹的氨基酸序列和抗原结构,开发适用于我国生殖器疱疹的基因工程疫苗有重要意义.

关键词: 疱疹病毒2型, 糖蛋白D, 聚合酶链反应, 核酸序列

Abstract: Objective To study the relation between the clinical feature and glycoprotein D gene sequence analysis of a wild strain of HSV 2 isolated from one relapsed patient with genital herpes.Methods The partial glycoprotein D gene sequence of the above mentioned strain was amplified and cloned with PCR.Results The comparison of the amino acid sequence of gD gene between the wild strain and HSV 2G strain published showed that there was a mutation at site80 and site159.Conclusion In order to develop effective vaccine which is suitable for China,it is necessary to investigate the differences of the gene structure and function of gD among HSV isolated from China and other countries.

Key words: Herpes simplex virus-2, Glycoprotein D, Polymerase chain reaction, Sequence analysis