中华皮肤科杂志 ›› 1998, Vol. 31 ›› Issue (3): 144-146.

• 论著 • 上一篇    下一篇

人乳头瘤病毒18型E6E7反义RNA表达重组体的构建

郭庆1, 曾凡钦1, 吕凌2, 王斌2   

  1. 1. 中山医科大学孙逸仙纪念医院皮肤科 广州 510120;
    2. 中山医科大学孙逸仙纪念医院分子医学研究中心 广州 510120
  • 收稿日期:1997-07-03 修回日期:1997-10-02 出版日期:1998-06-15 发布日期:1998-06-15

Construction of Human Papillomavirus Type 18 E6E7 Antisense RNA Expressing Recombinants

Guo Qing1, Zeng Fanqin1, Lü Ling2   

  1. Department of Dermatology, Memorial Hospital of Sun Yat Sen University of Medical Sciences, Guangzhou 510120
  • Received:1997-07-03 Revised:1997-10-02 Online:1998-06-15 Published:1998-06-15

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摘要： 目的 为了探讨人乳头瘤病毒(HPV)的致病机理和寻找由HPV所致疾病的治疗途径,研究了HPV18型E6E7反义RNA表达重组体的构建.方法 以HPV18型全基因质粒为模板,聚合酶链反应扩增出HPV18型E6E7区816bp片段,以逆转录病毒pLNSX为载体,构建HPV18型E6E7逆转录病毒重组体,并以限制性内切酶酶切和Southern杂交分别鉴定插入方向和特异性检测.结果和结论 筛选出可表达HPV18型E6E7逆转录病毒重组体.该反义RNA表达重组体的构建为进一步研究E6E7基因的作用,以及探索是否能通过反义技术调控E6E7基因的表达打下基础.

关键词: HPV18E6E7, 逆转录病毒载体, 反义RNA

Abstract: Objective In order to study the pathogenesis of human papillomavirus(HPV) and seek for a therapeutic approach of the diseases caused by HPV,the construction of HPV18 E6E7 antisense RNA expressing recombinants was studied.Methods We amplified the HPV18 E6E7 816bp by PCR with HPV18 plasmid DNA as the template.pLNSX retroviruses were used as vectors,the HPV18 E6E7 retrovirus recombinants were constructed.And then the recombinants were cleaved with restriction endonuclease and hybridized with Southern blot for identifying the inserting direction and special check respectively.Results and conclusion The HPV18 E6E7 antisense RNA retrovirus expressing recombinants were screened and obtained,which had laid the foundation of studying the function of E6E7 genes further and explore whether the antisense technique can adjust and control the expression of E6E7 genes.

Key words: HPV18E6E7, Retrovirus vector, Antisense RNA