中华皮肤科杂志 ›› 1998, Vol. 31 ›› Issue (3): 141-143.

• 论著 • 上一篇    下一篇

差别聚合酶链反应定量检测艾滋病患者外周血前病毒DNA水平

范雪莉1, 徐文严1, 范江1, 李子仁1, 闫小君2, 陈若延1   

  1. 1. 南京中国医学科学院、中国协和医科大学皮肤病研究所南京 210042;
    2. 全军基金诊断技术应用研究所
  • 收稿日期:1997-07-10 修回日期:1997-11-23 出版日期:1998-06-15 发布日期:1998-06-15

Differential Polymerase Chain Reaction for Quantitation of HIV DNA in Peripheral Blood Mononuclear Cell of AIDS Patients

Fan Xueli1, Xu Wenyan1, Fan Jiang1   

  1. Institute of Dermatology, Chinese Academy of Medical Sciences, Peking Union Medical College, Nanjing 210042
  • Received:1997-07-10 Revised:1997-11-23 Online:1998-06-15 Published:1998-06-15

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摘要： 目的 为了评价艾滋病的治疗效果、筛选有效治疗药物,了解患者体内病情变化,常常需要对治疗前后患者体内病毒水平进行客观的定量.方法 采用差别聚合酶链反应,共同扩增待测靶基因HIV-1gag和参照模板βglobin基因,建立起检测HIV-1复制水平的定量方法,并对8例艾滋病患者外周血前病毒DNA含量进行定量.结果 8例患者的前病毒DNA水平介于0.508～2.210拷贝数/μgDNA之间,其复制和整合水平存在差异.结论 该方法具有快速、可靠、重复性好等优点,非常适合于典型的临床标本的检测,为艾滋病疗效判定提供客观依据.

关键词: 聚合酶链反应, 差别, 艾滋病, 前病毒

Abstract: Objective In order to evaluate the therapeutic effect,to screen effective drugs for AIDS,and to find out the changes of patient's condition,it is necessary to develop an objective method to quantitate the levels of viral load of the patients before and after treatment.Method Differential PCR(D-PCR) was used to co-amplify the target HIV-1 gag gene and reference template β-globin gene,so as to determine the level of HIV replication quantitatively.The levels of HIV DNA in the peripheral blood mononuclear cells of 8 patients with AIDS were determined quantitatively.Results The levels of the provirus of the 8 patients ranged from 0.508-2.210 copies/μg DNA,there were differences in the levels of replication and integration.Conclusion It is the authors' opinion that this method is easy to control with an advantage of reliability,quickness and reproducibility,It is suitable to measure clinical specimens,and provides an objective evidence for the evaluation of responses to therapeutic intervention.

Key words: PCR, differential, AIDS, Provirus