中华皮肤科杂志 ›› 1997, Vol. 30 ›› Issue (5): 315-317.

• 论著 • 上一篇    下一篇

中波紫外线照射对人角朊细胞表达Ro/SSA抗原的影响

徐子刚, 张建中, 秦俭, 马圣清   

  1. 北京医科大学第一临床医学院皮肤科 100034
  • 收稿日期:1996-10-08 修回日期:1997-04-09 出版日期:1997-10-15 发布日期:1997-10-15

Effect of Ultraviolet B Irradiation on the Expression of Ro/SSA Antigen on Cultured Human Keratinocytes

Xu Zigang, Zhang Jianzhong, Qin Jian   

  1. Department of Dermatology, First Teaching Hospital, Beijing Medical University, Beijing 100034
  • Received:1996-10-08 Revised:1997-04-09 Online:1997-10-15 Published:1997-10-15

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摘要： 为了探讨紫外线诱发和加重皮肤红斑狼疮的机理,利用体外培养的人角朊细胞,通过间接免疫荧光法检测UVB照射对角朊细胞表达Ro/SSA抗原的影响.结果表明:角朊细胞在接受不同剂量UVB(50mJ/cm2,100mJ/cm2,200mJ/cm2)照射后细胞膜出现Ro/SSA抗原表达,并与UVB剂量有相关性,200mJ/cm2的UVB照射可使10.7%的角朊细胞膜表达Ro/SSA抗原.角朊细胞若先与10-5mol/L17-β-雌二醇孵育48小时再接受UVB照射(200mJ/cm2),结果较单纯接受相同剂量UVB照射的角朊细胞更易表达Ro/SSA抗原,提示UVB和雌激素对角朊细胞表达Ro/SSA抗原有协同作用.基于上述结果,推测UVB诱发的角朊细胞膜表达Ro/SSA抗原是诱发和加重光敏性红斑狼疮损害的一个重要因素.

关键词: UVB, 角朊细胞, Ro/SSA

Abstract: In order to investigate the mechanism of ultraviolet in triggering and exacerbating cutaneous lupus erythematosus,we conducted an in vitro experiment to study the effect of UVB irradiaton on the expression of Ro/SSA antigen on cultured human keratinocytes by indirect immunofluorenscence(IIF).The results revealed that the treatment of keratinocytes with serial doses of UVB (50mJ/cm2,100mJ/cm2,200mJ/cm2) induced Ro/SSA antigen expression on the cell membrane.The Ro/SSA expression showed a dose dependent pattern with the maximal expression of 10 7% at the dose of 200mJ/cm2.When 10-5 mol/L 17 β estradiol was added to the culture medium 48 hours prior to 200mJ/cm2 UVB exposure,it significantly increased Ro/SSA expression (17 2%) as compared with that induced by UVB alone(10.7%),which reveals that UVB irradiaton and estradiol treatment may bave synergetic action on the expression of Ro/SSA.From these results,we speculate that Ro/SSA expression on the surface of keratinocytes induced by UVB may function as an important trigger for antibody dependent keratinocyte damage in photosensitive cutaneous lupus erythematosus.

Key words: UVB, Keratinocyte, Ro/SSA